Monoclonal anti-mouse laminin antibodies:: AL-1 reacts with laminin α1 chain, AL-2 with laminin β1 chain, and AL-4 with the coiled-coil domain of laminin β1 chain

We analyzed the reactivity of three different commercially available rat monoclonal antibodies raised against mouse laminin-a1 beta 1 gamma 1 (laminin-11), AL-1, AL-2, and AL-4. Using ELISA assays, Western blot analysis and inummostainings we present refined epitope maps for these three laminin monoclonals. AL-1 reacted, as predicted with laminin alpha 1 chain. AL-4 has also been marketed as an alpha 1 chain specific probe, but we show here that AL-4 detects mouse laminin beta 1 chain, in the distal part of the coiled-coil region. AL-2 was predicted to react with all three chains near the cross-region, but seems to primarily react with laminin beta 1 chain. (c) 2006 Elsevier B.V./Intemational Society of Matrix Biology. All rights reserved.