Optimization of culture conditions and characterization of a new lectin from Aspergillus niger

被引：14
|
作者：
Singh, Ram Sarup [1 ]
Thakur, Gulshan [1 ]
Bhari, Ranjeeta [1 ]
机构：
[1] Punjabi Univ, Dept Biotechnol, Carbohydrate & Prot Biotechnol Lab, Patiala 147002, Punjab, India
来源：
INDIAN JOURNAL OF MICROBIOLOGY | 2009年 / 49卷 / 03期
关键词：
Aspergillus niger; Hemagglutination; Sugar specificity; Minimum inhibitory concentration (MIC); PURIFICATION; FUCOSE;
D O I：
10.1007/s12088-009-0041-x
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Lectin activity was determined on solidified medium containing agar and in broth cultures of Aspergillus niger. The fungus was found to express 16 times higher activity in broth cultures, when grown in a medium adjusted to pH 5.5 at 30A degrees C under stationary condition. Lectin activity was found to be expressed by 6-day-old mycelial cultures with maximum activity being expressed on 9th day of incubation. The crude lectin (total titer 1280) was found to be precipitated at 50% saturation of ammonium sulphate with 2.4-fold purifi cation and 83% yield in the precipitate. The partially purifi ed lectin was found to agglutinate all human, rat, mice and pig erythrocytes. It was found to have a strong binding affinity to mucin, asialofetuin and inulin.
引用
收藏
页码：219 / 222
页数：4
相关论文
共 50 条
  • [1] Optimization of culture conditions and characterization of a new lectin from Aspergillus niger
    Ram Sarup Singh
    Gulshan Thakur
    Ranjeeta Bhari
    Indian Journal of Microbiology, 2009, 49 : 219 - 222
  • [2] Optimization of culture conditions, partial purification and characterization of a new lectin from Aspergillus nidulans
    Singh, Ram Sarup
    Bhari, Ranjeeta
    Tiwary, Ashok Kumar
    ROMANIAN BIOTECHNOLOGICAL LETTERS, 2010, 15 (01): : 4990 - 4999
  • [3] Optimization of culture conditions for the production of an extracellular ribonuclease by Aspergillus niger in a benchtop bioreactor
    Xiong, YH
    Liu, JZ
    Song, HY
    Ji, LN
    WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, 2004, 20 (09): : 935 - 939
  • [4] Optimization of culture conditions for the production of an extracellular ribonuclease by Aspergillus niger in a benchtop bioreactor
    Ya-Hong Xiong
    Jian-Zhong Liu
    Hai-Yuan Song
    Liang-Nian Ji
    World Journal of Microbiology and Biotechnology, 2004, 20 : 935 - 939
  • [5] Optimization of medium composition and culture conditions for invertase production by Aspergillus niger ATCC 20611
    Dinarvand, M.
    Arbakariya, B.
    Moeini, H.
    Ajdari, Z.
    Mousavi, S. Sadegh
    Nahavandi, R.
    MINERVA BIOTECNOLOGICA, 2012, 24 (04) : 135 - 140
  • [6] Enhanced production of extracellular ribonuclease from Aspergillus niger by optimization of culture conditions using response surface methodology
    Xiong, YH
    Liu, HZ
    Song, HY
    Ji, LN
    BIOCHEMICAL ENGINEERING JOURNAL, 2004, 21 (01) : 27 - 32
  • [7] Influence of Culture Conditions on the Biotransformation of (+)-Limonene by Aspergillus niger
    Garcia-Carnelli, Carlos
    Rodriguez, Paula
    Heinzen, Horacio
    Menendez, Filar
    ZEITSCHRIFT FUR NATURFORSCHUNG SECTION C-A JOURNAL OF BIOSCIENCES, 2014, 69 (1-2): : 61 - 67
  • [8] Characterization of four new antifungal yanuthones from Aspergillus niger
    Petersen, Lene M.
    Holm, Dorte K.
    Knudsen, Peter B.
    Nielsen, Kristian F.
    Gotfredsen, Charlotte H.
    Mortensen, Uffe H.
    Larsen, Thomas O.
    JOURNAL OF ANTIBIOTICS, 2015, 68 (03): : 201 - 205
  • [9] Characterization of four new antifungal yanuthones from Aspergillus niger
    Lene M Petersen
    Dorte K Holm
    Peter B Knudsen
    Kristian F Nielsen
    Charlotte H Gotfredsen
    Uffe H Mortensen
    Thomas O Larsen
    The Journal of Antibiotics, 2015, 68 : 201 - 205
  • [10] ISOLATION AND CHARACTERIZATION OF ASPERENONE A NEW PHENYLPOLYENE FROM ASPERGILLUS NIGER
    JEFFERSON, WE
    BIOCHEMISTRY, 1967, 6 (11) : 3479 - +
12345