Development of Rapid UV Spectrophotometric Methods for Estimation of Celecoxib and Acyclovir in Formulations

Simple, sensitive, accurate, precise and rapid ultraviolet (UV) spectrophotometric methods having linearity range of 2-20 mu g/ml and low detection limit (DL) & quantitation limit (QL) were developed for estimation of celecoxib and acyclovir respectively in pure form, its formulations, dissolution samples and stability samples. In case of celecoxib, solvent system employed was 0.1N HCl with 1% w/v sodium lauryl sulphate (SLS) and wavelength of detection (lambda(det)) was 255 nm, while in case of acyclovir 0.1N HCl (solvent) and lambda(det) of 257 nm were employed for analysis. The developed methods were used to estimate the total drug content in two commercially available oral formulations of celecoxib and acyclovir each and recovery studies were carried out. Sample recoveries in all formulations using the above two methods were in good agreement with their respective label claims, thus suggesting the validity of the methods and non-interference of formulation excipients in the estimation. The developed methods were found to be stability specific and were validated as per ICH guidelines-2005, USP-2000 and statistical methods.