Comparable virus inactivation by bovine or vegetable derived Tween 80 during solvent/detergent treatment

被引:15
作者
Seitz, H [1 ]
Blümel, J [1 ]
Schmidt, I [1 ]
Willkommen, H [1 ]
Löwer, J [1 ]
机构
[1] Paul Ehrlich Inst, D-63225 Langen, Germany
关键词
enveloped viruses; inactivation; S/D treatment; Tween; 80;
D O I
10.1006/biol.2002.0328
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A mixture of Tri-n-butyl phosphate (TNBP) and Polysorbate 80 (Tween 80) is often used for virus inactivation during the manufacture of medicinal products derived from human plasma. This procedure, known as solvent/detergent treatment, is of high effectiveness for inactivation of enveloped viruses. Tween 80 can be manufactured from bovine tallow or from vegetable material. As the bovine-derived Tween 80 is normally used for the solvent/detergent treatment, the question has been raised whether vegetable-derived Tween 80 can be applied as an alternative substance for the solvent/detergent treatment. Comparable inactivation studies were therefore performed using Vesicular Stomatitis Virus (VSV), Pseudo-rabiesvirus (PRV), Semliki Forest Virus (SFV) and Bovine Diarrhoea Virus (BVDV). In principle, no differences were observed in the effectiveness of the solvent/detergent treatment when bovine or vegetable-derived Tween 80 was used. The comparability in the efficiency of both detergents for virus inactivation was shown to be independent of solvent/detergent concentration, of temperature (160 C and 6degreesC vs. 27degreesC and 25degreesC) and protein concentration (10% and 5% human albumin). In summary, vegetable-derived Tween 80 is of the same effectiveness as bovine-derived Tween 80, when used for virus inactivation by the solvent/detergent treatment. (C) 2002 The International Association for Biologicals. Published by Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:197 / 205
页数:9
相关论文
共 15 条
[1]   Viral inactivation of intramuscular immune serum globulins [J].
Alonso, WR ;
Trukawinski, S ;
Savage, M ;
Tenold, RA ;
Hammond, DJ .
BIOLOGICALS, 2000, 28 (01) :5-15
[2]  
BIESERT L, 1996, CLIN EXP RHEUMATOL, V14, P47
[3]  
*COMM PROP MED PRO, NOT GUID PLASM DER M
[4]  
*COMM PROP MED PRO, NOT GUID VIR VAL STU
[5]   Improvement of virus safety of a S/D-treated factor VIII concentrate by additional dry heat treatment at 100 degrees C [J].
Dichtelmuller, H ;
Rudnick, D ;
Breuer, B ;
Kotitschke, R ;
Kloft, M ;
Darling, A ;
Watson, E ;
Flehmig, B ;
Lawson, S ;
Frosner, G .
BIOLOGICALS, 1996, 24 (02) :125-130
[6]   TRI(N-BUTYL) PHOSPHATE DETERGENT TREATMENT OF LICENSED THERAPEUTIC AND EXPERIMENTAL BLOOD DERIVATIVES [J].
EDWARDS, CA ;
PIET, MPJ ;
CHIN, S ;
HOROWITZ, B .
VOX SANGUINIS, 1987, 52 (1-2) :53-59
[7]   VIRUS VALIDATION OF PLASMA-DERIVED PRODUCTS PRODUCED BY PHARMACIA, WITH PARTICULAR REFERENCE TO IMMUNOGLOBULINS [J].
ERIKSSON, B ;
WESTMAN, L ;
JERNBERG, M .
BLOOD COAGULATION & FIBRINOLYSIS, 1994, 5 :S37-S44
[8]   INACTIVATION OF VIRUSES IN LABILE BLOOD DERIVATIVES .1. DISRUPTION OF LIPID-ENVELOPED VIRUSES BY TRI( NORMAL-BUTYL)PHOSPHATE DETERGENT COMBINATIONS [J].
HOROWITZ, B ;
WIEBE, ME ;
LIPPIN, A ;
STRYKER, MH .
TRANSFUSION, 1985, 25 (06) :516-522
[9]  
HOROWITZ B, 1992, BLOOD, V79, P826
[10]   INACTIVATION OF VIRUSES IN LABILE BLOOD DERIVATIVES .2. PHYSICAL METHODS [J].
HOROWITZ, B ;
WIEBE, ME ;
LIPPIN, A ;
VANDERSANDE, J ;
STRYKER, MH .
TRANSFUSION, 1985, 25 (06) :523-527