Analysis of Quantum Rod Diffusion by Polarized Fluorescence Correlation Spectroscopy

被引：8

作者：

Lee, Jaeran ^{[1
]}

Fujii, Fumihiko ^{[2
]}

Kim, Soo Yong ^{[3
]}

Pack, Chan-Gi ^{[4
]}

Kim, Sok Won ^{[1
]}

机构：

[1] Univ Ulsan, Dept Phys, Ulsan 680749, South Korea

[2] Osaka Univ, WPI Immunol Frontier Res Ctr, Suita, Osaka, Japan

[3] Korea Adv Inst Sci & Technol, Dept Phys, Taejon 305701, South Korea

[4] RIKEN, Cellular Informat Lab, Wako, Saitama 3510198, Japan

来源：

JOURNAL OF FLUORESCENCE | 2014年 / 24卷 / 05期

基金：

新加坡国家研究基金会;

关键词：

Polarization; Spectroscopy; Fluorescence; Luminescence; Correlators; ROTATIONAL DIFFUSION; TRANSLATIONAL DIFFUSION; LIVING CELLS; NANORODS; DOTS; FLUCTUATIONS; NANOCRYSTALS; BLINKING; MOBILITY; PROBES;

D O I：

10.1007/s10895-014-1367-2

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

To measure the polarization dependence of fluorescent probes, a confocal-microscope-based polarized fluorescence correlation spectroscopy system was developed, and the polarization dependence on the rotational diffusion of well-defined quantum rods (Qrods) was investigated and characterized. The rotational diffusion region of the Qrods was observed over a time range of less than 10(-5) s in a water solution, and the rotational diffusion parameters were extracted using a rotational diffusion model in which the viscosity of the solution media was varied. Our work demonstrated that polarized fluorescence correlation spectroscopy (FCS) is useful for investigating both the rotational and translational diffusion of fluorescent probes.

引用

页码：1371 / 1378

页数：8

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