Endothelial and hematopoietic cell fate of human embryonic stem cells originates from primitive endothelium with hemangioblastic properties

被引：280

作者：

Wang, LS

Li, L

Shojaei, F

Levac, K

Cerdan, C

Menendez, P

Martin, T

Rouleau, A

Bhatia, M

机构：

[1] John P Robarts Res Inst, Krembil Ctr Stem Cell Biol & Regenerat Med, London, ON N6A 5K8, Canada

[2] Univ Western Ontario, Dept Microbiol & Immunol, London, ON, Canada

来源：

IMMUNITY | 2004年 / 21卷 / 01期

基金：

加拿大健康研究院;

关键词：

D O I：

10.1016/j.immuni.2004.06.006

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The cellular organization and relationships among precursors that initiate embryonic angiogenesis and hematopoiesis in the human have yet to be characterized. Here, we identify a subpopulation of primitive endothelial-like cells derived from human embryonic stem cells (hESCs) that express PECAM-1, Flk-1, and VE-cadherin, but not CD45 (CD45(neg)PFV cells), and that are uniquely responsible for endothelial and hematopoietic development. Molecular profiling of CD45(neg)PFV cells is consistent with endothelial and hematopoietic competency. Clonal isolation demonstrates that the CD45(neg)PFV population includes bipotent cells with endothelial and hematopoietic capacity. We suggest that human hematopoiesis and endothelial maturation originate exclusively from a subset of embryonic endothelium that possesses hemangioblastic properties and offers a model system to study these lineage relationships in the human.

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收藏

页码：31 / 41

页数：11

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