Immunolocalization of NblA, a protein involved in phycobilisome turnover, during heterocyst differentiation in cyanobacteria

被引：9

作者：

de Alda, JAGO ^{[1
]}

Lichtlé, C ^{[1
]}

Thomas, JC ^{[1
]}

Houmard, J ^{[1
]}

机构：

[1] Ecole Normale Super, CNRS, FRE 2433, Dept Biol, F-75230 Paris 05, France

来源：

MICROBIOLOGY-SGM | 2004年 / 150卷

关键词：

D O I：

10.1099/mic.0.26992-0

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

In unicellular non-diazotrophic cyanobacteria, NblA is a small polypeptide required for phycobilisome degradation during macronutrient limitation. In the filamentous N-2-fixing Tolypothrix sp., a nblA gene (nblAl) lies upstream of the cpeBA operon that encodes phycoerythrin apoproteins. Using a specific anti-NblAl antibody it was found that in strains of Tolypothrix sp. NblAl abundance increases under nitrogen-limiting conditions but the protein is also present in cells grown in nitrogen-replete medium. Gold immunolabelling experiments showed that, upon a nitrogen shift-down, NblAl is preferentially located in the differentiated heterocysts, where O-2 evolution has to be shut off for nitrogenase to operate. The results lead to the proposal that NblAl is a necessary 'cofactor' but not the triggering factor that governs phycobilisome degradation in Tolypothrix sp.

引用

页码：1377 / 1384

页数：8

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