Feasibility and Relevance of Examining Lymphoblastoid Cell Lines to Study Role of microRNAs in Autism

被引：131
作者：
Talebizadeh, Zohreh [1 ,2 ]
Butler, Merlin G. [1 ,2 ]
Theodoro, Mariana F. [1 ,2 ]
机构：
[1] Childrens Mercy Hosp & Clin, Sect Med Genet & Mol Med, Kansas City, MO 64108 USA
[2] Univ Missouri, Sch Med, Kansas City, MO 64108 USA
来源：
AUTISM RESEARCH | 2008年 / 1卷 / 04期
关键词：
microRNA; autism; lymphoblastoid cell lines; differential expression;
D O I：
10.1002/aur.33
中图分类号：
B84 [心理学]; C [社会科学总论]; Q98 [人类学];
学科分类号：
03 ; 0303 ; 030303 ; 04 ; 0402 ;
摘要：
To assess the feasibility and relevance of using lymphoblastoid cell lines to study the role of noncoding RNAs in the etiology of autism, we evaluated global expression profiling of 470 mature human microRNAs from six subjects with autism compared with six matched controls. Differential expression (either higher or lower) for 9 of the 470 microRNAs was observed in our autism samples compared with controls. Potential target genes for these microRNAs were identified using computer tools, which included several autism susceptibility genes. Our preliminary results indicate microRNAs should be considered and evaluated in the etiology of autism. In addition, analysis of this class of noncoding RNAs in lymphoblastoid cells has the potential to reveal at least a subset of brain-related microRNAs implicated in autism. Subsequently, this model system should allow for detection of complex subtle changes in susceptibility genes/pathways contributing to autism.
引用
收藏
页码：240 / 250
页数：11
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