Unpicking the Secrets of African Swine Fever Viral Replication Sites

被引:17
作者
Aicher, Sophie-Marie [1 ,3 ,4 ]
Monaghan, Paul [2 ,5 ]
Netherton, Christopher L. [1 ]
Hawes, Philippa C. [2 ]
机构
[1] Pirbright Inst, African Swine Fever Vaccinol Grp, Ash Rd, Woking GU24 0NF, Surrey, England
[2] Pirbright Inst, Bioimaging, Ash Rd, Woking GU24 0NF, Surrey, England
[3] Inst Pasteur, Virus Sensing & Signaling Unit, Dept Virol, UMR3569 CNRS, F-75724 Paris, France
[4] Univ Paris, F-75724 Paris, France
[5] Australian Ctr Dis Preparedness, 5 Portarlington Rd, East Geelong, Vic 3220, Australia
来源
VIRUSES-BASEL | 2021年 / 13卷 / 01期
基金
英国生物技术与生命科学研究理事会;
关键词
African swine fever virus; viral factories; viral proteins; click IT chemistry; STED microscopy; membrane assembly intermediates; electron tomography; FATTY-ACID SYNTHASE; CRYO-EM STRUCTURE; ENDOPLASMIC-RETICULUM; PLASMA-MEMBRANE; ASSEMBLY SITES; VIRUS; DNA; BIOGENESIS; RUPTURE; PRECURSORS;
D O I
10.3390/v13010077
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
African swine fever virus (ASFV) is a highly contagious pathogen which causes a lethal haemorrhagic fever in domestic pigs and wild boar. The large, double-stranded DNA virus replicates in perinuclear cytoplasmic replication sites known as viral factories. These factories are complex, multi-dimensional structures. Here we investigated the protein and membrane compartments of the factory using super-resolution and electron tomography. Click IT chemistry in combination with stimulated emission depletion (STED) microscopy revealed a reticular network of newly synthesized viral proteins, including the structural proteins p54 and p34, previously seen as a pleomorphic ribbon by confocal microscopy. Electron microscopy and tomography confirmed that this network is an accumulation of membrane assembly intermediates which take several forms. At early time points in the factory formation, these intermediates present as small, individual membrane fragments which appear to grow and link together, in a continuous progression towards new, icosahedral virions. It remains unknown how these membranes form and how they traffic to the factory during virus morphogenesis.
引用
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页数:18
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