Expression and prognostic relevance of MAGE-A3 and MAGE-C2 in non-small cell lung cancer

被引：38
作者：
Chen, Xinfeng [1 ,2 ]
Wang, Liping [1 ,2 ]
Liu, Jinyan [1 ]
Huang, Lan [1 ,2 ]
Yang, Li [1 ,2 ]
Gao, Qun [1 ,2 ]
Shi, Xiaojuan [1 ,2 ]
Li, Jieyao [1 ,2 ]
Li, Feng [1 ,2 ]
Zhang, Zhen [1 ]
Zhao, Song [3 ]
Zhang, Bin [4 ]
Van der Bruggen, Pierre [5 ]
Zhang, Yi [1 ,2 ,6 ,7 ]
机构：
[1] Zhengzhou Univ, Affiliated Hosp 1, Biotherapy Ctr, 1 Jianshe East Rd, Zhengzhou 450052, Henan, Peoples R China
[2] Zhengzhou Univ, Affiliated Hosp 1, Dept Oncol, Zhengzhou 450052, Henan, Peoples R China
[3] Zhengzhou Univ, Affiliated Hosp 1, Dept Cerebral Surg, Zhengzhou 450052, Henan, Peoples R China
[4] Northwestern Univ, Feinberg Sch Med, Div Hematol Oncol, Chicago, IL 60611 USA
[5] Catholic Univ Louvain, Duve Inst, Canc Res Brussels Branch, Ludwig Inst, B-1200 Brussels, Belgium
[6] Zhengzhou Univ, Sch Life Sci, Zhengzhou 450052, Henan, Peoples R China
[7] Engn Key Lab Cell Therapy Henan, Zhengzhou 450052, Henan, Peoples R China
来源：
ONCOLOGY LETTERS | 2017年 / 13卷 / 03期
基金：
中国国家自然科学基金;
关键词：
non-small cell lung cancer; cancer-germline genes; epithelial-mesenchymal transition; prognostic biomarker; CYTOLYTIC T-LYMPHOCYTES; TESTIS ANTIGENS; FREQUENT EXPRESSION; GENE-EXPRESSION; TUMOR-ANTIGENS; NY-ESO-1; IMMUNOTHERAPY; SURVIVAL;
D O I：
10.3892/ol.2017.5665
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
Melanoma-associated antigen (MAGE)-A3 and MAGE-C2 are antigens encoded by cancer-germline genes, and have been recognized as potential prognostic biomarkers and attractive targets for immunotherapy in multiple types of cancer. The present study aimed to analyze the clinicopathological significance of MAGE-A3/C2 expression in non-small cell lung cancer (NSCLC). The association between MAGE-A3/C2 mRNA and protein expression, and the pathological characteristics and overall survival of patients with NSCLC was analyzed. In addition, the functional role of MAGE-A3 in human NSCLC cell line A549 was examined in vitro. MAGE-A3/C2 mRNA expression was identified in 73% (151/206) and 53% (109/206) of patients with NSCLC, respectively. MAGE-A3/C2 protein expression was identified in 58% (44/76) and 53% (40/76) of NSCLC cases, respectively. MAGE-A3 mRNA expression was observed to be associated with smoking history, disease stage and lymph node metastasis. However, no association was identified between MAGE-C2 mRNA expression and the clinicopathological characteristics of patients with NSCLC. MAGE-A3/C2-positive patients had a poorer survival rate compared with MAGE-A3/C2-negative patients. Multivariate analysis identified that MAGE-A3 expression may serve as an independent marker of poor prognosis in patients with NSCLC. Downregulation of MAGE-A3 mRNA expression in A549 cells resulted in lower migration and colony formation rates, and a higher amount of epithelial marker and lower amount of mesenchymal marker expression compared with the control group. These results indicate that MAGE-A3 serves a role in NSCLC cell metastasis through the induction of epithelial-mesenchymal transition. In conclusion, MAGE-A3 may serve as a diagnostic and prognostic biomarker for patients with NSCLC, due to its association with tumor progression and poor clinical outcome.
引用
收藏
页码：1609 / 1618
页数：10
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