Two-Photon Voltage Imaging of Spontaneous Activity from Multiple Neurons Reveals Network Activity in Brain Tissue

被引：15

作者：

Li, Binglun ^{[1
]}

Chavarha, Mariya ^{[2
]}

Kobayashi, Yuho ^{[1
]}

Yoshinaga, Satoshi ^{[3
]}

Nakajima, Kazunori ^{[3
]}

Lin, Michael Z. ^{[2
]}

Inoue, Takafumi ^{[1
]}

机构：

[1] Waseda Univ, Sch Adv Sci & Engn, Dept Life Sci & Med Biosci, Tokyo 1628480, Japan

[2] Stanford Univ, Sch Med, Dept Neurobiol, Stanford, CA 94305 USA

[3] Keio Univ, Sch Med, Dept Anat, Tokyo 1608582, Japan

来源：

ISCIENCE | 2020年 / 23卷 / 08期

基金：

日本学术振兴会;

关键词：

LONG-TERM; MOUSE; PLASTICITY; CALCIUM; ELECTROPORATION; CONNECTIVITY; POPULATIONS; MICROSCOPY; SYNCHRONY; INDICATOR;

D O I：

10.1016/j.isci.2020.101363

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Recording the electrical activity of multiple neurons simultaneously would greatly facilitate studies on the function of neuronal circuits. The combination of the fast scanning by random-access multiphoton microscopy (RAMP) and the latest two-photon-compatiblehigh-performance fluorescent genetically encoded voltage indicators (GEVIs) has enabled action potential detection in deep layers in in vivo brain. However, neuron connectivity analysis on optically recorded action potentials from multiple neurons in brain tissue has yet to be achieved. With high expression of a two-photon-compatible GEVI, ASAP3, via in utero electroporation and RAMP, we achieved voltage recording of spontaneous activities from multiple neurons in brain slice. We provide evidence for the developmental changes in intralaminar horizontal connections in somatosensory cortex layer 2/3 with a greater sensitivity than calcium imaging. This method thus enables investigation of neuronal network connectivity at the cellular resolution in brain tissue.

引用

页数：33

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