Fatal systemic toxoplasmosis in Valley quail (Callipepla californica)

被引：15

作者：

Casagrande, Renata A. ^{[1
]}

Pena, Hilda F. J. ^{[2
]}

Cabral, Aline D. ^{[2
]}

Rolim, Veronica M. ^{[1
]}

de Oliveira, Luiz G. S. ^{[1
]}

Boabaid, Fabiana M. ^{[1
]}

Wouters, Angelica T. B. ^{[1
]}

Wouters, Flademir ^{[1
]}

Cruz, Clÿudio E. F. ^{[1
]}

Driemeier, David ^{[1
]}

机构：

[1] Univ Fed Rio Grande do Sul FaVet UFRGS, Fac Vet, Dept Patol Clin, Ave Bento Goncalves 9090, BR-91540000 Porto Alegre, RS, Brazil

[2] Univ Sao Paulo, Dept Med Vet Prevent & Saude Anim, Fac Med Vet & Zootecnia, Ave Orlando Marques de Paiva,87 Cidade Univ, BR-05508900 Sao Paulo, SP, Brazil

来源：

INTERNATIONAL JOURNAL FOR PARASITOLOGY-PARASITES AND WILDLIFE | 2015年 / 4卷 / 02期

关键词：

Captive bird; Genotyping; Immunohistochemistry; Pathology; Toxoplasma gondii;

D O I：

10.1016/j.ijppaw.2015.04.003

中图分类号：

Q14 [生态学（生物生态学）];

学科分类号：

071012 ; 0713 ;

摘要：

An adult, captive raised male Valley quail (Callipepla californica) acquired by a southern Brazilian aviary suddenly showed severe apathy, dyspnea and diarrhea, and died 18 hours after the onset of illness. At necropsy, pale muscles and whitish areas in the heart, splenomegaly, hepatomegaly, and consolidated red lungs were observed. Histological findings were mainly mononuclear inflammation with necrosis of liver, heart, spleen, bone marrow and lung. There were large numbers of Toxoplasma gondii tachyzoitesorganisms in the liver, heart, spleen, bone marrow, lungs, trachea, kidneys, adrenal glands, testes, intestines, and pancreas. These organisms were seen free in the organs' stroma or within macrophages and stained positively with polyclonal antiserum to T. gondii. Genomic DNA was extracted from the tissues and PCR was used to target the B1 gene of T. gondii. The genotypic characterization by PCR-RFLP with 11 markers (SAG1, SAG2 and alt. SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, Apico and CS3) revealed the ToxoDB-PCR-RFLP # 87 genotype, the same as previously identified in a backyard chicken (TgCkBr156) in Rio Grande do Sul, Brazil. (C) 2015 The Authors. Published by Elsevier Ltd on behalf of Australian Society for Parasitology.

引用

页码：264 / 267

页数：4

共 33 条

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