Molecular Characterization of a Novel Species of Capillovirus from Japanese Apricot (Prunus mume)

被引:14
作者
Marais, Armelle [1 ]
Faure, Chantal [1 ]
Theil, Sebastien [1 ]
Candresse, Thierry [1 ]
机构
[1] Univ Bordeaux, INRA, UMR Biol Fruit & Pathol 1332, CS 20032, F-33882 Villenave Dornon, France
来源
VIRUSES-BASEL | 2018年 / 10卷 / 04期
关键词
Next Generation Sequencing; fruit tree viruses; Betaflexiviridae; Mume virus A; Capillovirus; GENOME ORGANIZATION; NUCLEOTIDE-SEQUENCE; 1ST REPORT; LEAF; VIRUSES; DISEASE; GENUS; SPOT;
D O I
10.3390/v10040144
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
With the increased use of high-throughput sequencing methods, new viruses infecting Prunus spp. are being discovered and characterized, especially in the family Betaflexiviridae. Double-stranded RNAs from symptomatic leaves of a Japanese apricot (Prunus mume) tree from Japan were purified and analyzed by Illumina sequencing. Blast comparisons of reconstructed contigs showed that the P. mume sample was infected by a putative novel virus with homologies to Cherry virus A (CVA) and to the newly described Currant virus A (CuVA), both members of genus Capillovirus. Completion of the genome showed the new agent to have a genomic organization typical of capilloviruses, with two overlapping open reading frames encoding a large replication-associated protein fused to the coat protein (CP), and a putative movement protein (MP). This virus shares only, respectively, 63.2% and 62.7% CP amino acid identity with the most closely related viruses, CVA and CuVA. Considering the species demarcation criteria in the family and phylogenetic analyses, this virus should be considered as representing a new viral species in the genus Capillovirus, for which the name of Mume virus A is proposed.
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页数:8
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