Fatty Acid-Specific Alterations in Leptin, PPARα, and CPT-1 Gene Expression in the Rainbow Trout

被引:43
作者
Coccia, Elena [1 ]
Varricchio, Ettore [1 ]
Vito, Pasquale [1 ,2 ]
Turchini, Giovanni Mario [3 ]
Francis, David Scott [4 ]
Paolucci, Marina [1 ,5 ]
机构
[1] Dept Sci & Technol, I-82100 Benevento, Italy
[2] Biogem Consortium, I-83031 Ariano Irpino, Italy
[3] Deakin Univ, Sch Life & Environm Sci, Warrnambool, Vic, Australia
[4] Australian Inst Marine Sci, Townsville, Qld 4810, Australia
[5] ISA CNR, I-83100 Avellino, Italy
关键词
Fatty acids; Leptin; PPAR alpha; CPT-1; Rainbow trout; Liver; FISH-OIL REPLACEMENT; PROLIFERATOR-ACTIVATED RECEPTORS; CONJUGATED LINOLEIC-ACID; ONCORHYNCHUS-MYKISS; DOCOSAHEXAENOIC ACID; LONG-CHAIN; GENOME DUPLICATION; LIPID-METABOLISM; VEGETABLE-OIL; FOOD-INTAKE;
D O I
10.1007/s11745-014-3939-y
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
It is known that fatty acids (FA) regulate lipid metabolism by modulating the expression of numerous genes. In order to gain a better understanding of the effect of individual FA on lipid metabolism related genes in rainbow trout (Oncorhynchus mykiss), an in vitro time-course study was implemented where twelve individual FA (butyric 4:0; caprylic 8:0; palmitic (PAM) 16:0; stearic (STA) 18:0; palmitoleic16:1n-7; oleic 18:1n-9; 11-cis-eicosenoic 20:1n-9; linoleic (LNA) 18:2n-6; alpha-linolenic (ALA) 18:3n-3; eicosapentenoic (EPA) 20:5n-3; docosahexaenoic (DHA) 22:6n-3; arachidonic (ARA) 20:4n-6) were incubated in rainbow trout liver slices. The effect of FA administration over time was evaluated on the expression of leptin, PPAR alpha and CPT-1 (lipid oxidative related genes). Leptin mRNA expression was down regulated by saturated fatty acids (SFA) and LNA, and was up regulated by monounsaturated fatty acids (MUFA) and long chain PUFA, whilst STA and ALA had no effect. PPAR alpha and CPT-1mRNA expression were up regulated by SFA, MUFA, ALA, ARA and DHA; and down regulated by LNA and EPA. These results suggest that there are individual and specific FA induced modifications of leptin, PPAR alpha and CPT-1 gene expression in rainbow trout, and it is envisaged that such results may provide highly valuable information for future practical applications in fish nutrition.
引用
收藏
页码:1033 / 1046
页数:14
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