Nonsense-mediated mRNA decay of the ferric and cupric reductase mRNAs FRE1 and FRE2 in Saccharomyces cerevisiae

被引：3

作者：

Peccarelli, Megan ^{[1
]}

Scott, Taylor D. ^{[1
]}

Kebaara, Bessie W. ^{[1
]}

机构：

[1] Baylor Univ, Dept Biol, One Bear Pl 97388, Waco, TX 76798 USA

来源：

FEBS LETTERS | 2019年 / 593卷 / 22期

关键词：

biometal homeostasis; FRE1-2; mRNA; mRNA decay; nonsense-mediated mRNA decay; Saccharomyces cerevisiae; GENOME-WIDE ANALYSIS; WILD-TYPE; YEAST; ASSOCIATION; TRANSCRIPTS; EXPRESSION; PATHWAYS; UPF1P; ACT;

D O I：

10.1002/1873-3468.13545

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The nonsense-mediated mRNA decay (NMD) pathway regulates mRNAs that aberrantly terminate translation. This includes aberrant mRNAs and functional natural mRNAs. Natural mRNA degradation by NMD is triggered by mRNA features and environmental cues. Saccharomyces cerevisiae encodes multiple proteins with ferric and cupric reductase activity. Here, we examined the regulation by NMD of two mRNAs, FRE1 and FRE2, encoding ferric and cupric reductases in S. cerevisiae. We found that FRE2 mRNAs are regulated by NMD under noninducing conditions and that the FRE2 3 '-UTR contributes to the degradation of the mRNAs by NMD. Conversely, FRE1 mRNAs are not regulated by NMD under comparable conditions. These findings suggest that regulation of functionally related mRNAs by NMD can be differential and conditional.

引用

页码：3228 / 3238

页数：11

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