Two types of gynogenetic blunt snout bream derived from different sperm

被引：28

作者：

Gong, Dingbin ^{[1
,2
]}

Xu, Lihui ^{[1
,2
]}

Wu, Chang ^{[1
,2
]}

Wang, Shi ^{[1
,2
]}

Liu, Qingfeng ^{[1
,2
]}

Cao, Liu ^{[1
,2
]}

Mao, Zhuangwen ^{[1
,2
]}

Wang, Yude ^{[1
,2
]}

Hu, Fangzhou ^{[1
,2
]}

Zhou, Rong ^{[1
,2
]}

Zhang, Chun ^{[1
,2
]}

Tao, Min ^{[1
,2
]}

Luo, Kaikun ^{[1
,2
]}

Zhao, Rurong ^{[1
,2
]}

Wang, Yuequn ^{[1
,2
]}

Liu, Shaojun ^{[1
,2
]}

机构：

[1] Hunan Normal Univ, State Key Lab Dev Biol Freshwater Fish, Changsha 410081, Hunan, Peoples R China

[2] Hunan Normal Univ, Coll Life Sci, Changsha 410081, Hunan, Peoples R China

来源：

AQUACULTURE | 2019年 / 511卷

基金：

中国国家自然科学基金;

关键词：

Gynogenesis; Microsatellites; Blunt snout bream; Koi carp; Topmouth culter; Allo-sperm "hybrid" effect; Hypoxia tolerance; Cold shock; JAPANESE CRUCIAN CARP; COMMON CARP; MICROSATELLITE ANALYSIS; ARTIFICIAL GYNOGENESIS; MEIOTIC GYNOGENESIS; INDUCTION; HYPOXIA; HYBRIDS; GENES; EXPRESSION;

D O I：

10.1016/j.aquaculture.2019.734250

中图分类号：

S9 [水产、渔业];

学科分类号：

0908 ;

摘要：

Blunt snout bream (Megalobrama amblycephala, 2n = 48, BSB) is an important fish species with great commercial value but has a shortcoming in hypoxia tolerance. Gynogenesis is an effective way to obtain improved BSB with a higher ability to tolerate hypoxia. In this study, BSB eggs were activated by UV-irradiated sperm of koi carp (Cyprinus carpio haematopterus, 2n = 100, KOC) and topmouth culter (Erythroculter ilishaeformis, 2n = 48, TC), respectively. Following cold shock to double the chromosomes, two types of gynogenetic BSB (abbreviated as GBSB-K and GBSB-T) were obtained. Comparative analyses regarding the morphological characteristics, DNA content and chromosome number showed no significant difference among BSB, GBSB-K and GBSB-T (p > 0.05). However, the results of microsatellites (Cca15-K, Cca15-T) revealed that paternal DNA fragments were detected in GBSB-K and GBSB-T. These special DNA fragments were valuable molecular markers to distinguish gynogenetic BSB from BSB. Furthermore, the experimental results showed that GBSB-K and GBSB-T showed obviously higher hypoxia-tolerant ability than BSB (p < 0.05). Both KOC and TC were effective stimuli to induce gynogenesis of BSB and to form the "hybrid" effect. Factors including cold shock, barely living irradiated sperm and the allo-sperm "hybrid" effect potentially produce stronger gynogenetic BSB with greater hypoxia-tolerance ability. The formation of the two types of gynogenetic BSB derived from the two types of allo-sperm is of great significance in fish genetic breeding.

引用

页数：9

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