A single tube RT-PCR assay for the detection of mosquito-borne flaviviruses

被引：57
作者：
Ayers, M.
Adachi, D.
Johnson, G.
Andonova, M.
Drebot, M.
Tellier, R.
机构：
[1] Hosp Sick Children, Div Microbiol, Toronto, ON M5G 1X8, Canada
[2] Hosp Sick Children, Metabol Res Program, Toronto, ON M5G 1X8, Canada
[3] Hosp Sick Children, Div Microbiol, Toronto, ON, Canada
[4] Natl Microbiol Lab, Winnipeg, MB, Canada
来源：
JOURNAL OF VIROLOGICAL METHODS | 2006年 / 135卷 / 02期
关键词：
mosquito-borne flaviviruses; West Nile virus; RT-PCR;
D O I：
10.1016/j.jviromet.2006.03.009
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Mosquito-borne flaviviruses include several important agents of human disease and have provided striking examples of emerging infections. In this study we present the design and validation of a single tube RT-PCR assay using a pair of consensus primers for the detection of mosquito-borne flaviviruses. Sequencing of the amplicons permits the species identification. The assay was validated using RNA from the yellow fever virus vaccine strain and from representative strains of dengue viruses 1, 2, 3 and 4, West Nile virus, Kunjin virus (a clade of West Nile virus), and St. Louis encephalitis virus. (c) 2006 Elsevier B.V. All rights reserved.
引用
收藏
页码：235 / 239
页数：5
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