Polyacetylenes from Radix et Rhizoma Notopterygii Incisi with an Inhibitory Effect on Nitric Oxide Production In Vitro

被引：27

作者：

Blunder, Martina ^{[1
]}

Liu, Xin ^{[1
]}

Kunert, Olaf ^{[2
]}

Winkler, Nora Anna ^{[1
]}

Schinkovitz, Andreas ^{[1
]}

Schmiderer, Corinna ^{[3
]}

Novak, Johannes ^{[3
]}

Bauer, Rudolf ^{[1
]}

机构：

[1] Graz Univ, Dept Pharmacognosy, Inst Pharmaceut Sci, A-8010 Graz, Austria

[2] Graz Univ, Inst Pharmaceut Sci, Dept Pharmaceut Chem, A-8010 Graz, Austria

[3] Univ Vet Med, Inst Anim Nutr Funct Plant Cpds, Vienna, Austria

来源：

PLANTA MEDICA | 2014年 / 80卷 / 05期

基金：

奥地利科学基金会;

关键词：

Notopterygium incisum; Apiaceae; polyacetylenes; nitric oxide; iNOS; Griess assay; DNA sequencing; ACETYLENIC-COMPOUNDS; CONSTITUENTS; AUTHENTICATION; FALCARINDIOL; EXPRESSION; SYNTHASE; EXTRACT; ROOTS;

D O I：

10.1055/s-0034-1368196

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Notopterygium roots (Qiang Huo) have been used in traditional Chinese medicine for treating colds, inflammatory diseases like rheumatoid arthritis, and as an analgesic. The anti-inflammatory activity of the roots of Notopterygium incisum has been evaluated by testing the inhibitory activity on nitric oxide production by inducible nitric oxide synthase. The apparent authenticity of the sample was checked by DNA sequence comparison. Using activity-guided isolation, different compounds were isolated and structurally characterized by means of NMR and mass spectroscopy. Eight polyacetylenes could be identified and were tested on their inhibitory activity on nitric oxide production in RAW 264.7 mouse macrophages using the Griess assay. Different 3-hydroxy allyl polyacetylenes exhibited significant activity (IC50: 8-acetoxyfalcarinol, 20.1 mu M; falcarindiol, 9.2 mu M; 9-epoxyfalcarindiol, 8.8 mu M; and crithmumdiol, 23.6 mu M).

引用

页码：415 / 418

页数：4

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