Isolation and characterization of microsatellites in Brassica rapa L.

被引：162

作者：

Suwabe, K ^{[1
]}

Iketani, H ^{[1
]}

Nunome, T ^{[1
]}

Kage, T ^{[1
]}

Hirai, M ^{[1
]}

机构：

[1] NIVTS, Ageo, Mie 5142392, Japan

来源：

THEORETICAL AND APPLIED GENETICS | 2002年 / 104卷 / 6-7期

关键词：

Brassica rapa; microsatellites; molecular marker; segregation analysis; simple sequence repeats;

D O I：

10.1007/s00122-002-0875-7

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

We report here the isolation and characterization of microsatellites, or simple sequence repeats (SSRs), in Brassica rapa. The size-fractionated genomic library was screened with (GA)(15) and (GT)(15) oligonucleotide probes. A total of 58 clones were identified as having the microsatellite repeats, and specific primer pairs were designed for 38 microsatellite loci. All primer pairs, except two, amplified fragments having the sizes expected from the sequences. Of the 36 primer pairs, 35 amplified polymorphic loci in 19 cultivars of B. rapa, while monomorphism was observed in only one primer pair. A total of 232 alleles was identified by the 36 primer pairs in 19 cultivars of B. rapa, and these primer pairs were examined also in nine Brassicaceae species. Most of the 36 primer pairs amplified the loci in the Brassicaceae species. Segregation of the microsatellites was studied in an F-2 population from a cross of doubled-haploid lines DH27 x G309. The microsatellites segregated in a co-dominant manner. These results indicate that the microsatellites isolated in this study were highly informative and could be useful tools for genetic analysis in B. rapa and other related species.

引用

页码：1092 / 1098

页数：7

共 34 条

[1] Identification and mapping of a quantitative trait locus controlling extreme late bolting in Chinese cabbage (Brassica rapa L. ssp pekinensis syn. campestris L.) using bulked segregant analysis -: A QTL controlling extreme late bolting in Chinese cabbage [J].