Household siblings and nasal and fecal microbiota in infants

被引:25
作者
Hasegawa, Kohei [1 ]
Linnemann, Rachel W. [2 ]
Mansbach, Jonathan M. [3 ]
Ajami, Nadim J. [4 ]
Espinola, Janice A. [1 ]
Fiechtner, Lauren G. [5 ]
Petrosino, Joseph F. [4 ]
Camargo, Carlos A., Jr. [1 ]
机构
[1] Harvard Med Sch, Massachusetts Gen Hosp, Dept Emergency Med, Boston, MA USA
[2] Boston Childrens Hosp, Dept Med, Boston, MA USA
[3] MassGen Hosp Children, Div Gastroenterol & Nutr & Gen Acad Pediat, Boston, MA USA
[4] Emory Univ, Sch Med, Dept Pediat, Atlanta, GA USA
[5] Baylor Coll Med, Dept Mol Virol & Microbiol, Alkek Ctr Metagen & Microbiome Res, Houston, TX 77030 USA
基金
美国国家卫生研究院;
关键词
airway microbiota; asthma; fecal microbiota; infant; sibling; GUT MICROBIOTA; INTESTINAL MICROBIOTA; EARLY-CHILDHOOD; ASTHMA; PATTERNS; DISEASE; HYGIENE; COHORTS; NUMBER; ECZEMA;
D O I
10.1111/ped.13168
中图分类号
R72 [儿科学];
学科分类号
100202 ;
摘要
BackgroundEarly-life exposure to older siblings is associated with a lower risk of asthma. To date, no study has addressed the impact of having siblings on both the airway and fecal microbiota during infancy. The aim of this study was therefore to profile the nasal airway and fecal microbiota in infants, and to examine the association between having siblings and microbiota profile. MethodsWe conducted a cross-sectional study of 105 healthy infants (aged <1 year). Using 16S rRNA gene sequencing and an unbiased clustering approach to the nasal airway and fecal samples, we identified microbiota profiles and then determined the association between having siblings and microbiome profile. ResultsOverall, the median age was 3.4 months (IQR, 2.0-4.7 months); 43% had siblings in the household. Unbiased clustering of nasal airway microbiota identified three profiles: Moraxella dominant (43%), Corynebacterium/Dolosigranulum dominant (36%), and mixed (21%). Infants with siblings were more likely to have a Moraxella-dominant profile than Corynebacterium/Dolosigranulum-dominant profile (76% vs 18%), while those without siblings had the opposite pattern (18% vs 50%; P < 0.001, multivariable-adjusted). Fecal microbiota consisted of three profiles: Bifidobacterium dominant (39%), Escherichia dominant (31%), and Enterobacter dominant (30%). Infants with siblings were more likely to have a Bifidobacterium-dominant profile than Escherichia-dominant profile (49% vs 24%) while those without siblings had the opposite pattern (32% vs 37%; P = 0.04, multivariable-adjusted). ConclusionsIn this cross-sectional study, infants with siblings were more likely to have a Moraxella-dominant nasal microbiota profile and Bifidobacterium-dominant fecal microbiota profile. These findings should facilitate further investigation of the interplay between early-life environmental exposure, the microbiome, and childhood asthma.
引用
收藏
页码:473 / 481
页数:9
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