Regeneration of flowering plants from difficile lily protoplasts by means of a nurse culture

被引：35

作者：

Horita, M ^{[1
]}

Morohashi, H ^{[1
]}

Komai, F ^{[1
]}

机构：

[1] Hokkaido Green Bio Inst, Naganuma, Hokkaido 0691317, Japan

来源：

PLANTA | 2002年 / 215卷 / 05期

关键词：

Lilium; nurse culture; oriental hybrids; plant regeneration; protoplast;

D O I：

10.1007/s00425-002-0849-7

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

The regeneration of difficile lily protoplasts isolated from suspension cells of the Oriental hybrid lily (Lilium L.) cultivars Casablanca, Siberia and Acapulco was achieved by using the nurse-culture method. The divided protoplasts grew into colonies with nurse cells that have no regeneration ability, and developed to visible calli on a medium containing picloram. Many plantlets were formed on the calli after transfer of the proliferated calli to hormone-free medium. We were able to transplant the plantlets to soil in pots without acclimatization, and the plantlets grew in a greenhouse until flowering 2 years later.

引用

页码：880 / 884

页数：5

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