Confocal Raman spectroscopy to monitor intracellular penetration of TiO2 nanoparticles

被引：13

作者：

Salehi, Hamideh ^{[1
]}

Calas-Bennasar, Isabelle ^{[1
]}

Durand, Jean-Cedric ^{[1
]}

Middendorp, Elodie ^{[1
]}

Valcarcel, Jean ^{[1
]}

Larroque, Christian ^{[2
]}

Nagy, Katalin ^{[3
]}

Turzo K, Kinga ^{[3
]}

Dekany, Imre ^{[4
]}

Cuisinier, Frederic J. G. ^{[1
]}

机构：

[1] Univ Montpellier I, Lab Biol Sante Nanosci, UFR Odontol, EA 4203, F-34193 Montpellier, France

[2] Univ Montpellier I, Ctr Reg Lutte Canc Val dAurelle Paul Lamarque, IRCM INSERM896, F-34298 Montpellier, France

[3] Univ Szeged, Fac Dent, H-6720 Szeged, Hungary

[4] Hungarian Acad Sci, MTA SZTE Supramol & Nanostruct Mat Res Grp, H-6720 Szeged, Hungary

来源：

JOURNAL OF RAMAN SPECTROSCOPY | 2014年 / 45卷 / 09期

关键词：

titanium dioxide; Raman microscopy; K-mean cluster; correlation coefficient; cell imaging; TITANIUM-DIOXIDE NANOPARTICLES; SCANNING-ELECTRON-MICROSCOPY; OXIDE NANOPARTICLES; IN-VITRO; CELLS; APOPTOSIS; MITOCHONDRIA; TOXICITY; DNA;

D O I：

10.1002/jrs.4561

中图分类号：

O433 [光谱学];

学科分类号：

0703 ; 070302 ;

摘要：

Confocal Raman microscopy, a noninvasive, label-free, and high-spatial resolution imaging technique, in combination with K-mean cluster analysis and a correlation coefficient map, was employed to trace titanium dioxide (TiO2) nanoparticles in living MCF-7 and TERT cells. The penetration of TiO2 nanoparticles into cells revealed a gradual time-dependent diffusion of nanoparticles over the entire cell. Cell apoptosis was monitored by tracing cytochrome c diffusion into the cytoplasm. A comparison with the mitochondrial clustering indicated that cytochrome c was inside the mitochondria for TiO2 concentration of 2 mu gml(-1). This result demonstrates that the presence of TiO2 particles within a cell does not induce apoptosis. We demonstrated that confocal Raman microscopy allow to follow penetration of TiO2 particles in cell and to monitor the apoptotic status of the penetrated cells. Copyright (c) 2014 John Wiley & Sons, Ltd.

引用

页码：807 / 813

页数：7

共 52 条

[1] Evolution of mitochondrial gene content: gene loss and transfer to the nucleus [J].