Different roles of Numb-p72 and Numb-p65 on the trafficking of metabotropic glutamate receptor 5

被引：3

作者：

Wang, Na ^{[1
]}

Wang, Dan-Dan ^{[2
]}

Hou, Xiaolin ^{[2
]}

Li, Xiumao ^{[3
]}

Shen, Ying ^{[4
]}

机构：

[1] Zhejiang Univ City Coll, Sch Med, Hangzhou, Zhejiang, Peoples R China

[2] NingXia Med Univ, Gen Hosp, Yinchuan, Ningxia, Peoples R China

[3] Zhejiang Univ, Affiliated Hosp 2, Dept Orthoped, Sch Med, Hangzhou, Zhejiang, Peoples R China

[4] Zhejiang Univ, Dept Physiol, Sch Med, Hangzhou, Zhejiang, Peoples R China

来源：

MOLECULAR BIOLOGY REPORTS | 2021年 / 48卷 / 01期

基金：

中国国家自然科学基金;

关键词：

Numb; Metabotropic glutamate receptor; Internalization; Trafficking; Numb-p72; AMYLOID PRECURSOR PROTEIN; DIVISION; REVEALS; COMPLEX; DOMAIN;

D O I：

10.1007/s11033-020-06103-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We previously reported that Numb, a protein localized to clathrin-coated vesicles, regulates the membrane expression of metabotropic glutamate receptor 5 (mGluR5) and is critical to social behaviors. However, the distinct actions of Numb isoforms on mGluR5 have not been investigated. Here, we showed that the expression patterns of Numb-p72 and Numb-p65, two important isoforms of Numb, were distinct in HEK293T cells. Numb-p72, but not Numb-p65, bound to mGluR5 alpha, and enhanced mGluR5 membrane expression by inhibiting its internalization. Our results suggest that a complete structure is required for Numb to bind to mGluR5 and to modulate mGluR5 trafficking.

引用

页码：595 / 600

页数：6

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