Pathogenicity and molecular characteristics of infectious bronchitis virus (IBV) strains isolated from broilers showing diarrhoea and respiratory disease

被引:22
作者
Chacon, J. L. [1 ]
Assayag, M. S., Jr. [1 ]
Revolledo, L. [1 ]
Astolfi-Ferreira, C. S. [1 ]
Vejarano, M. P. [1 ]
Jones, R. C. [2 ]
Piantino Ferreira, A. J. [1 ]
机构
[1] Univ Sao Paulo, Sch Vet Med, Dept Pathol, BR-05508900 Sao Paulo, Brazil
[2] Univ Liverpool, Inst Global Hlth, Dept Infect Biol, Liverpool CH64 7TE, Merseyside, England
关键词
POLYMERASE-CHAIN-REACTION; LAYING HENS; REDUCED FERTILITY; GENETIC DIVERSITY; SPIKE PROTEIN; IN-VIVO; S1; GENE; CHICKENS; PROTECTION; SEROTYPES;
D O I
10.1080/00071668.2014.903558
中图分类号
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号
0905 ;
摘要
1. The possibility that infectious bronchitis virus (IBV) variants isolated from broilers with enteric and respiratory problems have a different tropism and pathological outcome from those IBV strains causing classical respiratory disease was investigated.2. IBV variants were isolated from broiler flocks with enteric and respiratory problems in two regions of Brazil. The USP-10 isolate, of enteric origin, was inoculated via the oral oroculonasal routes into IBV-antibody-free broilers and specific pathogen-free (SPF) chickens to determine tissue tropism and pathogenicity and compared with an IBV variant (USP-50) isolated from chickens showing signs of respiratory disease only.3. Both USP-10 and USP-50 strains caused similar pathological patterns by either route of inoculation. Both variants were detected in respiratory and non-respiratory tissues, including the kidney, intestine and testis.4. Broilers were more susceptible to infection than SPF chickens, and seroconversion was detected in all of the chicks.
引用
收藏
页码:271 / 283
页数:13
相关论文
共 45 条
[1]   EARLY PATHOGENESIS IN CHICKS OF INFECTION WITH AN ENTEROTROPIC STRAIN OF INFECTIOUS-BRONCHITIS VIRUS [J].
AMBALI, AG ;
JONES, RC .
AVIAN DISEASES, 1990, 34 (04) :809-817
[2]  
Benyeda Z., 2010, J COMP PATHOL, V134, P1
[3]   Comparison of the pathogenicity of QX-like, M41 and 793/B infectious bronchitis strains from different pathological conditions [J].
Benyeda, Zsofia ;
Mato, Tamas ;
Sueveges, Tibor ;
Szabo, Eva ;
Kardi, Veronika ;
Abonyi-Toth, Zsolt ;
Rusvai, Miklos ;
Palya, Vilmos .
AVIAN PATHOLOGY, 2009, 38 (06) :449-456
[4]   Molecular epizootiology of avian infectious bronchitis in Russia [J].
Bochkov, Yury A. ;
Batchenko, Galina V. ;
Shcherbakova, Lidiya O. ;
Borisov, Alexander V. ;
Drygin, Vladimir V. .
AVIAN PATHOLOGY, 2006, 35 (05) :379-393
[5]   Avian infectious bronchitis virus: A possible cause of reduced fertility in the rooster [J].
Boltz, DA ;
Nakai, M ;
Bahr, JM .
AVIAN DISEASES, 2004, 48 (04) :909-915
[6]   Epididymal stone formation and decreased sperm production in roosters vaccinated with a killed strain of avian infectious bronchitis virus [J].
Boltz, David A. ;
Zimmerman, Claire R. ;
Nakai, Masaaki ;
Bunick, David ;
Scherba, Gail ;
Bahr, Janice M. .
AVIAN DISEASES, 2006, 50 (04) :594-598
[7]   Recombinant avian infectious bronchitis virus expressing a heterologous spike gene demonstrates that the spike protein is a determinant of cell tropism [J].
Casais, R ;
Dove, B ;
Cavanagh, D ;
Britton, P .
JOURNAL OF VIROLOGY, 2003, 77 (16) :9084-9089
[8]   Relationship between sequence variation in the S1 spike protein of infectious bronchitis virus and the extent of cross-protection in vivo [J].
Cavanagh, D ;
Ellis, MM ;
Cook, JKA .
AVIAN PATHOLOGY, 1997, 26 (01) :63-74
[9]   Coronaviruses from pheasants (Phasianus colchicus) are genetically closely related to coronaviruses of domestic fowl (infectious bronchitis virus) and turkeys [J].
Cavanagh, D ;
Mawditt, K ;
Welchman, DD ;
Britton, P ;
Gough, RE .
AVIAN PATHOLOGY, 2002, 31 (01) :81-93
[10]   Longitudinal field studies of infectious bronchitis virus and avian pneumovirus in broilers using type-specific polymerase chain reactions [J].
Cavanagh, D ;
Mawditt, K ;
Britton, P ;
Naylor, CJ .
AVIAN PATHOLOGY, 1999, 28 (06) :593-605