Lipid Directed Intrinsic Membrane Protein Segregation

被引：42

作者：

Hansen, Jesper S. ^{[1
]}

Thompson, James R. ^{[1
]}

Helix-Nielsen, Claus ^{[2
]}

Malmstadt, Noah ^{[1
]}

机构：

[1] Univ So Calif, Mork Family Dept Chem Engn & Mat Sci, Los Angeles, CA 90089 USA

[2] Tech Univ Denmark, Dept Phys, DTU Phys, DK-2800 Lyngby, Denmark

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2013年 / 135卷 / 46期

基金：

美国国家卫生研究院;

关键词：

GIANT UNILAMELLAR VESICLES; DROPLET INTERFACE BILAYERS; TRANSMEMBRANE PROTEINS; PHASE-SEPARATION; MODEL MEMBRANES; RECONSTITUTION; CHANNELS; LIPOSOMES; MIXTURES; BEHAVIOR;

D O I：

10.1021/ja409708e

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We demonstrate a new approach for direct reconstitution of membrane proteins during giant vesicle formation. We show that it is straightforward to create a tissue-like giant vesicle film swelled with membrane protein using aquaporin SoPIP2;1 as an illustration. These vesicles can also be easily harvested for individual study. By controlling the lipid composition we are able to direct the aquaporin into specific immiscible liquid domains in giant vesicles. The oligomeric a-helical protein cosegregates with the cholesterol-poor domains in phase separating ternary mixtures.

引用

页码：17294 / 17297

页数：4

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