Molybdenum Trioxide Nanoparticles with Intrinsic Sulfite Oxidase Activity

被引：138

作者：

Ragg, Ruben ^{[1
]}

Natalio, Filipe ^{[2
]}

Tahir, Muhammad Nawaz ^{[1
]}

Janssen, Henning ^{[3
]}

Kashyap, Anubha ^{[3
]}

Strand, Dennis ^{[3
]}

Strand, Susanne ^{[3
]}

Tremel, Wolfgang ^{[1
]}

机构：

[1] Johannes Gutenberg Univ Mainz, Inst Anorgan Chem & Analyt Chem, D-55099 Mainz, Germany

[2] Univ Halle Wittenberg, Inst Chem Anorgan Chem, D-06120 Halle, Germany

[3] Johannes Gutenberg Univ Mainz, Med Klin & Poliklin, Univ Med, D-55131 Mainz, Germany

来源：

ACS NANO | 2014年 / 8卷 / 05期

关键词：

enzyme mimic; molybdenum oxide; sulfite oxidase; sulfite oxidase deficiency; nanoparticles; catalysis; biomimetics; MOO3; PURIFICATION; OXIDATION; CATALYSTS; TUNGSTEN; MODEL;

D O I：

10.1021/nn501235j

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Sulfite oxidase is a mitochondria-located molybdenum-containing enzyme catalyzing the oxidation of sulfite to sulfate in the amino acid and lipid metabolism. Therefore, it plays a major role in detoxification processes, where defects in the enzyme cause a severe infant disease leading to early death with no efficient or cost-effective therapy in sight. Here we report that molybdenum trioxide (MoO3) nanoparticles display an intrinsic biomimetic sulfite oxidase activity under physiological conditions, and, functionalized with a customized bifunctional ligand containing dopamine as anchor group and triphenylphosphonium ion as targeting agent, they selectively target the mitochondria while being highly dispersible in aqueous solutions. Chemically induced sulfite oxidase knockdown cells treated with MoO3 nanoparticles recovered their sulfite oxidase activity in vitro, which makes MoO3 nanoparticles a potential therapeutic for sulfite oxidase deficiency and opens new avenues for cost-effective therapies for gene-induced deficiencies.

引用

页码：5182 / 5189

页数：8

共 42 条

[1]

Ahmad A, 2008, INDIAN J BIOCHEM BIO, V45, P379

[2]

Andre R., 2013, NEW FUTURE DEV CATAL, P149

[3] V2O5 Nanowires with an Intrinsic Peroxidase-Like Activity [J].

Andre, Rute ;

Natalio, Filipe ;

Humanes, Madalena ;

Leppin, Jana ;

Heinze, Katja ;

Wever, Ron ;

Schroeder, H. -C. ;

Mueller, Werner E. G. ;

Tremel, Wolfgang .

ADVANCED FUNCTIONAL MATERIALS, 2011, 21 (03) :501-509

[4] Direct conversion of methane into methanol over MoO3/SiO2 catalyst in an excess amount of water vapor [J].

Aoki, K ;

Ohmae, M ;

Nanba, T ;

Takeishi, K ;

Azuma, N ;

Ueno, A ;

Ohfune, H ;

Hayashi, H ;

Udagawa, Y .

CATALYSIS TODAY, 1998, 45 (1-4) :29-33

[5] MOLYBDENUM COFACTOR DEFICIENCY [J].

ARNOLD, GL ;

GREENE, CL ;

STOUT, JP ;

GOODMAN, SI .

JOURNAL OF PEDIATRICS, 1993, 123 (04) :595-598

[6] CONVERSION OF CHOLESTANOL TO 12-KETOCHOLESTANOL AND TO DELTA14-CHOLESTENOL AND DELTA8(14)-CHOLESTENOL BY REMOTE OXIDATION [J].

BRESLOW, R ;

BALDWIN, SW .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1970, 92 (03) :732-&

[7] Sulfite oxidase protects plants against sulfur dioxide toxicity [J].

Brychkova, Galina ;

Xia, Zongliang ;

Yang, Guohua ;

Yesbergenova, Zhazira ;

Zhang, Zhili ;

Davydov, Olga ;

Fluhr, Robert ;

Sagi, Moshe .

PLANT JOURNAL, 2007, 50 (04) :696-709

[8] Semitelechelic HPMA copolymers functionalized with triphenylphosphonium as drug carriers for membrane transduction and mitochondrial localization [J].

Callahan, Jon ;

Kopecek, Jindrich .

BIOMACROMOLECULES, 2006, 7 (08) :2347-2356

[9] Rare earth nanoparticles prevent retinal degeneration induced by intracellular peroxides [J].

Chen, Junping ;

Patil, Swanand ;

Seal, Sudipta ;

McGinnis, James F. .

NATURE NANOTECHNOLOGY, 2006, 1 (02) :142-150

[10]

COHEN HJ, 1971, J BIOL CHEM, V246, P359

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