Multiplex real-time PCR assay for Legionella species

被引：5
|
作者：
Kim, Seung Min [1 ]
Jeong, Yoojung [3 ]
Sohn, Jang Wook [2 ,3 ]
Kim, Min Ja [1 ,2 ,3 ]
机构：
[1] Korea Univ, Coll Med, Dept Med, Seoul 136705, South Korea
[2] Korea Univ, Coll Med, Div Infect Dis, Dept Internal Med, Seoul 136705, South Korea
[3] Korea Univ, Coll Med, Inst Emerging Infect Dis, Seoul 136705, South Korea
来源：
MOLECULAR AND CELLULAR PROBES | 2015年 / 29卷 / 06期
基金：
新加坡国家研究基金会;
关键词：
Legionnaires' disease; Legionella species; Multiplex; Real-time PCR; COMMUNITY-ACQUIRED PNEUMONIA; LEGIONNAIRES-DISEASE; PNEUMOPHILA; SPP; DIFFERENTIATION; SPECIMENS; EPIDEMIC; GENOMICS; SEQUENCE; TARGETS;
D O I：
10.1016/j.mcp.2015.08.004
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Legionella pneumophila serogroup 1 (sg1) accounts for the majority of infections in humans, but other Legionella species are also associated with human disease. In this study, a new SYBR Green I-based multiplex real-time PCR assay in a single reaction was developed to allow the rapid detection and differentiation of Legionella species by targeting specific gene sequences. Candidate target genes were selected, and primer sets were designed by referring to comparative genomic hybridization data of Legionella species. The Legionella species-specific groES primer set successfully detected all 30 Legionella strains tested. The xcpX and ribA primers specifically detected L. pneumophila sg1-15 and L pneumophila sg1, respectively. In addition, this assay was validated by testing clinical samples and isolates. In conclusion, this novel multiplex real-time PCR assay might be a useful diagnostic tool for the rapid detection and differentiation of Legionella species in both clinical and epidemiological studies. (C) 2015 Elsevier Ltd. All rights reserved.
引用
收藏
页码：414 / 419
页数：6
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