A label-free electrochemical immunosensor based on Au@Pd/Ag yolk-bimetallic shell nanoparticles and amination graphene for detection of nuclear matrix protein 22

被引：69

作者：

Li, Na ^{[1
]}

Wang, Yulan ^{[1
]}

Li, Yueyun ^{[2
]}

Cao, Wei ^{[1
]}

Ma, Hongmin ^{[1
]}

Wu, Dan ^{[1
]}

Du, Bin ^{[1
]}

Wei, Qin ^{[1
]}

机构：

[1] Univ Jinan, Sch Chem & Chem Engn, Key Lab Chem Sensing & Anal Univ Shandong, Jinan 250022, Peoples R China

[2] Shandong Univ Technol, Sch Chem Engn, Zibo 255049, Peoples R China

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 2014年 / 202卷

基金：

中国国家自然科学基金;

关键词：

Label-free immunosensor; Amination graphene; Yolk-shell nanoparticles; Nuclear matrix protein 22; HUMAN BLADDER-CANCER; IMPEDIMETRIC IMMUNOSENSOR; GOLD NANOPARTICLES; DIAGNOSIS; NMP22; CORE; IMMUNOASSAY; ELECTRODE; NANOSTRUCTURES; MICROSPHERES;

D O I：

10.1016/j.snb.2014.05.077

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A sensitive label-free electrochemical immunosensor was developed based on Au@Pd/Ag yolk-bimetallic shell nanoparticles (Au@Pd/Ag NPs) and amination graphene (NH2-GS) for the detection of nuclear matrix protein 22 (NMP22). Au@Pd/Ag NPs were loaded on the surface of NH2-GS (NH2-GS/Au@Pd/Ag) by covalent binding between noble metal NPs and amino groups. A glassy carbon electrode (GCE, 4 mm in diameter) was used as the working electrode for the assembly of NPs and NH2-GS. Due to the large specific surface area and excellent electrical conductivity of NH2-GS/Au@Pd/Ag, the electroactivity of K-3[Fe(CN)(6)] as electron transfer mediator was promoted and the amount of NMP22 antibody immobilized was enhanced significantly. Under optimum conditions, the immunosensor exhibited high selectivity and a wide linear range from 0.01 to 18 ng/mL with a detection limit of 3.3 pg/mL. For real sample analysis, the recovery of standard addition was 97.4-99.3% and the relative standard deviation (RSD) was 4.83-7.21%, which indicated that the proposed method exhibited great potential as a reliable technique for the determination of NMP22 for clinical research and diagnostic applications. (C) 2014 Elsevier B.V. All rights reserved.

引用

页码：67 / 73

页数：7

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