Evaluation of viscosities of aqueous two-phase systems containing protein

被引：5

作者：

Gündüz, U ^{[1
]}

机构：

[1] Gazi Univ, Muhendislik Mimarlik Fak, TR-06570 Ankara, Turkey

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2004年 / 807卷 / 01期

关键词：

Grunberg equation; viscosity; aqueous two-phase systems; polyethylene glycol; bovine serum albumin;

D O I：

10.1016/j.jchromb.2004.01.048

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The dynamic viscosities of aqueous polyethylene glycol, aqueous bovine serum albumin, and polyethylene glycol-bovine serum albumin-water solutions were measured at temperatures of 15, 20, 25, 30 and 35 degreesC. To estimate the viscosity values of polyethylene glycol-bovine serum albumin-water solutions, a one parameter Grunberg-like model which was satisfactorily used earlier by the present author for polyethylene glycol-dextran-water solutions was employed. The disposable parameter a for our temperature range was estimated as 3.71. The relative errors varying from 0.29 to 18.98 in absolute value indicates that the Grunberg-like model works perfectly for polymer-protein solutions as well. (C) 2004 Elsevier B.V. All rights reserved.

引用

页码：157 / 161

页数：5

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