Multiphase Water-in-Oil Emulsion Droplets for Cell-Free Transcription-Translation

被引：79

作者：

Torre, Paola ^{[1
]}

Keating, Christine D. ^{[2
]}

Mansy, Sheref S. ^{[1
]}

机构：

[1] Univ Trento, CIBIO, I-38123 Mattarello, Italy

[2] Penn State Univ, Dept Chem, University Pk, PA 16802 USA

来源：

LANGMUIR | 2014年 / 30卷 / 20期

基金：

美国国家科学基金会;

关键词：

POLYMER ENCAPSULATION; PROTEINS; ORIGIN;

D O I：

10.1021/la404146g

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The construction of genetically encoded cellular mimics in compartments containing organized synthetic cytosols is desirable for the development of artificial cells. Phase separated aqueous domains were placed within water-in-oil emulsion droplets in a manner compatible with transcription and translation machinery. Aqueous two-phase and three-phase systems (ATPS and A3PS) were assembled with dextran, poly(ethylene glycol), and Ficoll. Aqueous two-phase systems were capable of supporting the cell-free expression of protein within water droplets, whereas the aqueous three-phase-based system did not give rise to detectable protein synthesis. The expressed protein preferentially partitioned to the dextran-enriched phase. The system could serve as a foundation for building cellular mimics with liquid organelles.

引用

页码：5695 / 5699

页数：5

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