Temporal Stability of Bacterial Communities in Antarctic Sponges

被引:28
作者
Cardenas, Cesar A. [1 ]
Font, Alejandro [1 ]
Steinert, Georg [2 ,3 ]
Rondon, Rodolfo [1 ]
Gonzalez-Aravena, Marcelo [1 ]
机构
[1] Inst Antartico Chileno, Dept Cient, Punta Arenas, Chile
[2] Carl von Ossietzky Univ Oldenburg, Inst Chem & Biol Marine Environm, Oldenburg, Germany
[3] GEOMAR Helmholtz Ctr Ocean Res Kiel, Marine Symbioses, Kiel, Germany
关键词
Porifera; cold-water sponges; Antarctica; WAP; 16S rRNA; environmental variability; MICROBIAL DIVERSITY; MARINE; PROFILES; TEMPERATURE; VARIABILITY; SPECIFICITY; SYMBIOSIS; DYNAMICS;
D O I
10.3389/fmicb.2019.02699
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Marine sponges host dense, diverse, and species-specific microbial communities around the globe; however, most of the current knowledge is restricted to species from tropical and temperate waters. Only recently, some studies have assessed the microbiome of a few Antarctic sponges; however, contrary to low mid-latitude sponges, the knowledge about temporal (stability) patterns in the bacterial communities of Antarctic sponges is absent. Here, we studied the temporal patterns of bacterial communities in the Antarctic sponges Mycale (Oxymycale) acerata, Isodictya sp., Hymeniacidon torquata, and Tedania (Tedaniopsis) wellsae that were tagged in situ and monitored during three austral summers over a 24-month period. By using amplicon sequencing of the bacterial 16S rRNA gene we found that the microbiome differed between species. In general, bacterial communities were dominated by gammaproteobacterial OTUs; however, M. acerata showed the most distinct pattern, being dominated by a single betaproteobacterial OTU. The analysis at OTU level (defined at 97% sequence similarity) showed a highly stable bacterial community through time, despite the abnormal seawater temperatures (reaching 3 degrees C) and rates of temperature increase of 0.15 degrees C day(-1) recorded in austral summer 2017. Sponges were characterized by a small core bacterial community that accounted for a high percentage of the abundance. Overall, no consistent changes in core OTU abundance were recorded for all studied species, confirming a high temporal stability of the microbiome. In addition, predicted functional pathway profiles showed that the most abundant pathways among all sponges belonged mostly to metabolism pathway groups (e.g., amino acid, carbohydrate, energy, and nucleotide). The predicted functional pathway patterns differed among the four sponge species. However, no clear temporal differences were detected supporting what was found in terms of the relatively stable composition of the bacterial communities.
引用
收藏
页数:14
相关论文
共 85 条
[1]  
Anderson M. J., 2008, Permanova+ for Primer: Guide to Software and Statistical Methods, P1
[2]   Spatial and temporal variability of the bacterial community in different chemotypes of the New Zealand marine sponge Mycale hentscheli [J].
Anderson, Sally A. ;
Northcote, Peter T. ;
Page, Michael J. .
FEMS MICROBIOLOGY ECOLOGY, 2010, 72 (03) :328-342
[3]  
ASSHAUER KP, 2015, BIOINFORMATICS, V31, P2882, DOI DOI 10.1093/BI0INF0RMATICS/BTV287
[4]   Spatial and temporal variation in shallow seawater temperatures around Antarctica [J].
Barnes, David K. A. ;
Fuentes, Veronica ;
Clarke, Andrew ;
Schloss, Irene R. ;
Wallace, Margaret I. .
DEEP-SEA RESEARCH PART II-TOPICAL STUDIES IN OCEANOGRAPHY, 2006, 53 (8-10) :853-865
[5]   Iceberg killing fields limit huge potential for benthic blue carbon in Antarctic shallows [J].
Barnes, David K. A. .
GLOBAL CHANGE BIOLOGY, 2017, 23 (07) :2649-2659
[6]   Specificity and temporal dynamics of complex bacteria-sponge symbiotic interactions [J].
Bjoerk, Johannes R. ;
Diez-Vives, C. ;
Coma, Rafel ;
Ribes, Marta ;
Montoya, Jose M. .
ECOLOGY, 2013, 94 (12) :2781-2791
[7]  
Borchert E, 2016, FRONT MICROBIOL, V7, DOI [10.3389/fmicb.2010.01027, 10.3389/fmicb.2016.01027]
[8]   Changes in the metabolic potential of the sponge microbiome under ocean acidification [J].
Botte, Emmanuelle S. ;
Nielsen, Shaun ;
Abdul Wahab, Muhammad Azmi ;
Webster, John ;
Robbins, Steven ;
Thomas, Torsten ;
Webster, Nicole S. .
NATURE COMMUNICATIONS, 2019, 10 (1)
[9]   High temporal variability in bacterial community, silicatein and hsp70 expression during the annual life cycle of Hymeniacidon sinapium (Demospongiae) in China's Yellow Sea [J].
Cao, Heng ;
Cao, Xupeng ;
Guan, Xiaoyan ;
Xue, Song ;
Zhang, Wei .
AQUACULTURE, 2012, 358 :262-273
[10]   High similarity in the microbiota of cold-water sponges of the Genus Mycale from two different geographical areas [J].
Cardenas, Cesar A. ;
Gonzalez-Aravena, Marcelo ;
Font, Alejandro ;
Hestetun, Jon T. ;
Hajdu, Eduardo ;
Trefault, Nicole ;
Malmbergg, Maja ;
Bongcarn-Rudloff, Erik .
PEERJ, 2018, 6