MotionCor2: anisotropic correction of beam-induced motion for improved cryo-electron microscopy

被引：5367

作者：

Zheng, Shawn Q. ^{[1
,2
]}

Palovcak, Eugene ^{[1
]}

Armache, Jean-Paul ^{[1
]}

Verba, Kliment A. ^{[1
]}

Cheng, Yifan ^{[1
,2
]}

Agard, David A. ^{[1
,2
]}

机构：

[1] Univ Calif San Francisco, Dept Biochem & Biophys, San Francisco, CA 94143 USA

[2] Univ Calif San Francisco, Howard Hughes Med Inst, San Francisco, CA 94143 USA

来源：

NATURE METHODS | 2017年 / 14卷 / 04期

关键词：

CRYO-EM;

D O I：

10.1038/nmeth.4193

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

MotionCor2 software corrects for beam-induced sample motion, improving the resolution of cryo-EM reconstructions.

引用

页码：331 / 332

页数：2

共 8 条

[1] Ribosome structures to near-atomic resolution from thirty thousand cryo-EM particles [J].

Bai, Xiao-chen ;

Fernandez, Israel S. ;

McMullan, Greg ;

Scheres, Sjors H. W. .

ELIFE, 2013, 2

[2] Beam-induced motion of vitrified specimen on holey carbon film [J].

Brilot, Axel F. ;

Chen, James Z. ;

Cheng, Anchi ;

Pan, Junhua ;

Harrison, Stephen C. ;

Potter, Clinton S. ;

Carragher, Bridget ;

Henderson, Richard ;

Grigorieff, Nikolaus .

JOURNAL OF STRUCTURAL BIOLOGY, 2012, 177 (03) :630-637

[3] Single-Particle Cryo-EM at Crystallographic Resolution [J].

Cheng, Yifan .

CELL, 2015, 161 (03) :450-457

[4] Measuring the optimal exposure for single particle cryo-EM using a 2.6 Å reconstruction of rotavirus VP6 [J].

Grant, Timothy ;

Grigorieff, Nikolaus .

ELIFE, 2015, 4

[5] The Resolution Revolution [J].

Kuehlbrandt, Werner .

SCIENCE, 2014, 343 (6178) :1443-1444

[6] Electron counting and beam-induced motion correction enable near-atomic-resolution single-particle cryo-EM [J].

Li, Xueming ;

Mooney, Paul ;

Zheng, Shawn ;

Booth, Christopher R. ;

Braunfeld, Michael B. ;

Gubbens, Sander ;

Agard, David A. ;

Cheng, Yifan .

NATURE METHODS, 2013, 10 (06) :584-+

[7] Structure of the TRPV1 ion channel determined by electron cryo-microscopy [J].

Liao, Maofu ;

Cao, Erhu ;

Julius, David ;

Cheng, Yifan .

NATURE, 2013, 504 (7478) :107-+

[8] Alignment of cryo-EM movies of individual particles by optimization of image translations [J].

Rubinstein, John L. ;

Brubaker, Marcus A. .

JOURNAL OF STRUCTURAL BIOLOGY, 2015, 192 (02) :188-195

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