Essential roles of Drosophila RhoA in the regulation of neuroblast proliferation and dendritic but not axonal morphogenesis

被引：245

作者：

Lee, TM ^{[1
]}

Winter, C ^{[1
]}

Marticke, SS ^{[1
]}

Lee, A ^{[1
]}

Luo, LQ ^{[1
]}

机构：

[1] Stanford Univ, Dept Biol Sci, Stanford, CA 94305 USA

来源：

NEURON | 2000年 / 25卷 / 02期

关键词：

D O I：

10.1016/S0896-6273(00)80896-X

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

The pleiotropic functions of small GTPase Rho present a challenge to its genetic analysis in multicellular organisms. We report here the use of the MARCM (mosaic analysis with a repressible cell marker) system to analyze the function of RhoA in the developing Drosophila brain. Clones of cells homozygous for null RhoA mutations were specifically labeled in the mushroom body (MB) neurons of mosaic brains. We found that RhoA is required for neuroblast (Nb) proliferation but not for neuronal survival. Surprisingly, RhoA is not required for MB neurons to establish normal axon projections. However, neurons lacking RhoA overextend their dendrites, and expression of activated RhoA causes a reduction of dendritic complexity. Thus, RhoA is an important regulator of dendritic morphogenesis, while distinct mechanisms are used for axonal morphogenesis.

引用

页码：307 / 316

页数：10

共 45 条

[1] The rho GTPase and a putative RhoGEF mediate a signaling pathway for the cell shape changes in Drosophila gastrulation [J].