Comparison of loop-mediated isothermal amplification (LAMP) and nested-PCR assay targeting the RE and B1 gene for detection of Toxoplasma gondii in blood samples of children with leukaemia

被引：58

作者：

Fallahi, Shirzad ^{[1
,2
]}

Tabaei, Seyyed Javad Seyyed ^{[2
]}

Pournia, Yadollah ^{[4
]}

Zebardast, Nozhat ^{[2
]}

Kazemi, Bahram ^{[2
,3
]}

机构：

[1] Lorestan Univ Med Sci, Dept Med Parasitol & Mycol, Khorramabad, Iran

[2] Shahid Beheshti Univ Med Sci, Dept Med Parasitol & Mycol, Tehran, Iran

[3] Shahid Beheshti Univ Med Sci, Dept Biotechnol, Tehran, Iran

[4] Lorestan Univ Med Sci, Fac Med, Khorramabad, Iran

来源：

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2014年 / 79卷 / 03期

关键词：

LAMP; Nested-PCR; RE; B1; Toxoplasma gondii; Children with leukaemia; POLYMERASE-CHAIN-REACTION; 529-BP REPEAT ELEMENT; TIME PCR; CEREBRAL TOXOPLASMOSIS; MOLECULAR DIAGNOSIS; CLINICAL-DIAGNOSIS; FALCIPARUM-MALARIA; QUANTITATIVE PCR; RAPID DETECTION; DNA;

D O I：

10.1016/j.diagmicrobio.2014.02.014

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Toxoplasmosis diagnosis constitutes an important measure for disease prevention and control. In this paper, a newly described DNA amplification technique, loop-mediated isothermal amplification (LAMP), and nested-PCR targeting the repeated element (RE) and B1 gene, were compared to each other for the detection of Toxoplasma gondii DNA in blood samples of children with leukaemia. One hundred ten blood samples from these patients were analyzed by LAMP and nested-PCR. Out of 50 seropositive samples (IgM+, IgG+), positive results were obtained with 92% and 86% on RE, B1-LAMP and 82% and 68% on RE, BI-nested PCR analyses, respectively. Of the 50 seronegative samples, three, two and one samples were detected positive by RE-LAMP, B1-LAMP and RE-nested PCR assays, respectively, while none were detected positive by BI-nested PCR. None of the 10 IgM-, IgG+ samples was detected positive after testing LAMP and nested-PCR assays in duplicate. This is the first report of a study in which the LAMP method was applied with high sensitivity and efficacy for the diagnosis of T. gonii in blood samples of children with leukaemia. (C) 2014 Elsevier Inc. All rights reserved.

引用

页码：347 / 354

页数：8

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