Determination and pharmacokinetic studies of arecoline in dog plasma by liquid chromatography-tandem mass spectrometry

被引:11
作者
Li, Bing [1 ,2 ,3 ]
Zhou, Xu-Zheng [1 ,2 ,3 ]
Li, Jian-Yong [1 ,2 ,3 ]
Yang, Ya-Jun [1 ,2 ,3 ]
Niu, Jian-Rong [1 ,2 ,3 ]
Wei, Xiao-Juan [1 ,2 ,3 ]
Liu, Xi-Wang [1 ,2 ,3 ]
Li, Jin-Shan [1 ,2 ,3 ]
Zhang, Ji-Yu [1 ,2 ,3 ]
机构
[1] Minist Agr, Key Lab Vet Pharmaceut Dev, Lanzhou, Peoples R China
[2] Key Lab New Anim Drug Project Gansu Prov, Lanzhou, Peoples R China
[3] CAAS, Lanzhou Inst Husb & Pharmaceut Sci, Lanzhou 730050, Peoples R China
来源
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2014年 / 969卷
关键词
Arecoline hydrobromide; Arecoline hydrobromide tablets; LC-MS/MS; Pharmacokinetics; Dog plasma; TAENIASIS/CYSTICERCOSIS;
D O I
10.1016/j.jchromb.2014.07.043
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A rapid and sensitive high-performance liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated for the determination of arecoline concentration in dog plasma. Plasma sample was prepared by protein precipitation using n-hexane (containing 1% isoamyl alcohol) with beta-pinene as an internal standard. Chromatographic separation was achieved on an Agilent C18 column (4.6 x 75 mm, 3.5 mu m) using methanol:5 mM ammonium acetate as the mobile phase with isocratic elution. Mass detection was carried out using positive electrospray ionization in multiple reaction monitoring mode. The calibration curve for arecoline was linear over a concentration range of 2-500 ng/mL. The intra-day and inter-day accuracy and precision were within the acceptable limits of +/- 10% at all concentrations. In summary, the LC-MS/MS method described herein was fully validated and successfully applied to the pharmacokinetic study of arecoline hydrobromide tablets in dogs after oral administration. (C) 2014 Published by Elsevier B.V.
引用
收藏
页码:12 / 18
页数:7
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