TREATMENT WITH CAULERPA MICROPHYSA PEPSIN-DIGESTED EXTRACT INDUCES APOPTOSIS IN MURINE LEUKEMIA WEHI-3 CELLS

被引：3
作者：
Chou, Su-Tze [1 ]
Lin, Hui-Chiu [2 ]
Chuang, Mei-Yu [1 ]
Chiu, Tsai-Hsin [3 ]
机构：
[1] Providence Univ, Dept Food & Nutr, Taichung, Taiwan
[2] Penghu Marine Biol Res Ctr, Fisheries Res Inst, Pingtung, Penghu County, Taiwan
[3] Natl Penghu Univ Sci & Technol, Dept Food Sci, Magong City 880, Penghu County, Taiwan
来源：
JOURNAL OF FOOD BIOCHEMISTRY | 2014年 / 38卷 / 05期
关键词：
ENDOPLASMIC-RETICULUM STRESS; MEMBRANE PERMEABILIZATION; OXIDATIVE STRESS; FLOW-CYTOMETRY; IN-VITRO; CANCER; DEATH; ACTIVATION; INDUCTION; ANTIOXIDANT;
D O I：
10.1111/jfbc.12079
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
In this study, we examined the possible apoptotic mechanism of pepsin-digested extract of Caulerpa microphysa (CME) in myelomonocytic leukemia (WEHI-3) cells. Flow cytometry demonstrated that CME induced cell cycle arrest in the G0/G1 phase and stimulated reactive oxygen species (ROS) production and calcium release but caused a loss of the mitochondrial membrane potential (MMP). The results indicated that the protein levels of cyclin D, cyclin E, CDK6, CDK2 and Bcl-2 decreased and those of p21, p27, p53, Bax, Bid, GRP78, GADD153, apoptosis-inducing factor (AIF), caspase-3 and caspase-9 increased in WEHI-3 cells after CME treatment. In conclusion, CME induced G0/G1 phase arrest, decreased MMP and increased Ca2+ release and ROS production in the WEHI-3 cells. The results suggest that CME may have potential as an anticancer agent.
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收藏
页码：469 / 479
页数：11
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