Molecular detection and genetic diversity of bovine Babesia spp., Theileria orientalis, and Anaplasma marginale in beef cattle in Thailand

被引：42

作者：

Jirapattharasate, Charoonluk ^{[1
,2
]}

Moumouni, Paul Franck Adjou ^{[1
]}

Cao, Shinuo ^{[1
]}

Iguchi, Aiko ^{[1
]}

Liu, Mingming ^{[1
]}

Wang, Guanbo ^{[1
]}

Zhou, Mo ^{[1
]}

Vudriko, Patrick ^{[1
]}

Efstratiou, Artemis ^{[1
]}

Changbunjong, Tanasak ^{[2
]}

Sungpradit, Sivapong ^{[2
]}

Ratanakorn, Parntep ^{[3
]}

Moonarmart, Walasinee ^{[3
]}

Sedwisai, Poonyapat ^{[4
]}

Weluwanarak, Thekhawet ^{[4
]}

Wongsawang, Witsanu ^{[5
]}

Suzuki, Hiroshi ^{[1
]}

Xuan, Xuenan ^{[1
]}

机构：

[1] Obihiro Univ Agr & Vet Med, Natl Res Ctr Protozoan Dis, Obihiro, Hokkaido 0808555, Japan

[2] Mahidol Univ, Dept Preclin & Appl Anim Sci, Fac Vet Sci, Salaya 73170, Nakhon Pathom, Thailand

[3] Mahidol Univ, Dept Clin Sci & Publ Hlth, Fac Vet Sci, Salaya 73170, Nakhon Pathom, Thailand

[4] Mahidol Univ, Monitoring & Surveillance Ctr Zoonot Dis Wildlife, Fac Vet Sci, Salaya 73170, Nakhon Pathom, Thailand

[5] Mahidol Univ, Livestock & Wildlife Hosp, Fac Vet Sci, Kanchanaburi Campus, Saiyok Dist 71150, Kanchanaburi, Thailand

来源：

PARASITOLOGY RESEARCH | 2017年 / 116卷 / 02期

关键词：

Babesia spp; Theileria orientalis; Anaplasma marginale; Phylogenetic tree; Beef cattle; Thailand; INTERNAL TRANSCRIBED SPACER; VECTOR-BORNE PATHOGENS; EPIDEMIOLOGIC SURVEY; HEMOLYTIC-ANEMIA; WATER-BUFFALOS; INFECTION; BIGEMINA; IDENTIFICATION; PREVALENCE; ARTHROPODS;

D O I：

10.1007/s00436-016-5345-2

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Babesia spp., Theileria orientalis, and Anaplasma marginale are significant tick-borne pathogens that affect the health and productivity of cattle in tropical and subtropical areas. In this study, we used PCR to detect the presence of Babesia bovis, Babesia bigemina, and T. orientalis in 279 beef cattle from Western Thailand and A. marginale in 608 beef cattle from the north, northeastern, and western regions. The PCRs were performed using species-specific primers based on the B. bovis spherical body protein 2 (BboSBP2), B. bigemina rhoptry-associated protein 1a (BbiRAP-1a), T. orientalis major piroplasm surface protein (ToMPSP), and A. marginale major surface protein 4 (AmMSP4) genes. To determine the genetic diversity of the above parasites, amplicons of B. bovis and B. bigemina ITS1-5.8s rRNA gene-ITS2 regions (B. bovis ITS, B. bigemina ITS), ToMPSP, and AmMSP4 genes were sequenced for phylogenetic analysis. PCR results revealed that the prevalence of B. bovis, B. bigemina, T. orientalis, and A. marginale in the Western region was 11.1, 12.5, 7.8, and 39.1 %, respectively. Coinfections of two or three parasites were observed in 17.9 % of the animals sampled. The study revealed that the prevalence of A. marginale in the western region was higher than in the north and northeastern regions (7 %). Sequence analysis showed the BboSBP2 gene to be more conserved than B. bovis ITS in the different isolates and, similarly, the BbiRAP-1a was more conserved than B. bigemina ITS. In the phylogenetic analysis, T. orientalis MPSP sequences were classified into types 3, 5, and 7 as previously reported. A. marginale MSP4 gene sequences shared high identity and similarity with each other and clustered with isolates from other countries. This study provides information on the prevalence and genetic diversity of tick-borne pathogens in beef cattle and highlights the need for effective strategies to control these pathogens in Thailand.

引用

页码：751 / 762

页数：12

共 43 条

[1] Development and evaluation of a nested PCR based on spherical body protein 2 gene for the diagnosis of Babesia bovis infection [J].