Charge Neutralization Drives the Shape Reconfiguration of DNA Nanotubes

被引：24

作者：

Liu, Pi ^{[1
,2
,3
]}

Zhao, Yan ^{[4
,5
]}

Liu, Xiaoguo ^{[4
,5
]}

Sun, Jixue ^{[1
,2
]}

Xu, Dede ^{[1
,2
]}

Li, Yang ^{[1
,2
]}

Li, Qian ^{[4
,5
]}

Wang, Lihua ^{[4
,5
]}

Yang, Sichun ^{[6
,7
]}

Fan, Chunhai ^{[4
,5
]}

Lin, Jianping ^{[1
,2
,3
]}

机构：

[1] Nankai Univ, Coll Pharm, State Key Lab Med Chem Biol, Tianjin 300353, Peoples R China

[2] Nankai Univ, Tianjin Key Lab Mol Drug Res, Tianjin 300353, Peoples R China

[3] Tianjin Inst Ind Biotechnol, Biodesign Ctr, Tianjin 300308, Peoples R China

[4] Chinese Acad Sci, Div Phys Biol, Shanghai 201800, Peoples R China

[5] Chinese Acad Sci, CAS Key Lab Interfacial Phys & Technol, Shanghai Synchrotron Radiat Facil, Shanghai Inst Appl Phys,Bioimaging Ctr, Shanghai 201800, Peoples R China

[6] Case Western Reserve Univ, Ctr Prote, 10900 Euclid Ave, Cleveland, OH 44106 USA

[7] Case Western Reserve Univ, Dept Nutr, 10900 Euclid Ave, Cleveland, OH 44106 USA

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2018年 / 57卷 / 19期

基金：

上海市自然科学基金; 国家重点研发计划; 美国国家科学基金会;

关键词：

DNA nanotechnology; molecular channels; molecular dynamics; SAXS; shape reconfiguration; X-RAY-SCATTERING; ORIGAMI NANOSTRUCTURES; MOLECULAR-DYNAMICS; CRYSTAL-STRUCTURE; CHANNEL; MEMBRANE; TRANSPORT; ELASTICITY; MECHANISMS;

D O I：

10.1002/anie.201801498

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Reconfiguration of membrane protein channels for gated transport is highly regulated under physiological conditions. However, a mechanistic understanding of such channels remains challenging owing to the difficulty in probing subtle gating-associated structural changes. Herein, we show that charge neutralization can drive the shape reconfiguration of a biomimetic 6-helix bundle DNA nanotube (6HB). Specifically, 6HB adopts a compact state when its charge is neutralized by Mg2+; whereas Na+ switches it to the expanded state, as revealed by MD simulations, small-angle X-ray scattering (SAXS), and FRET characterization. Furthermore, partial neutralization of the DNA backbone charges by chemical modification renders 6HB compact and insensitive to ions, suggesting an interplay between electrostatic and hydrophobic forces in the channels. This system provides a platform for understanding the structure-function relationship of biological channels and designing rules for the shape control of DNA nanostructures in biomedical applications.

引用

页码：5418 / 5422

页数：5

共 39 条

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