Development of a real-time PCR for Bartonella spp. detection, a current emerging microorganism

被引：11
作者：
Parra, Elena [1 ]
Segura, Ferran [1 ]
Tijero, Jessica [1 ]
Pons, Imma [1 ]
Nogueras, Maria-Mercedes [1 ]
机构：
[1] Univ Autonoma Barcelona, Corp Sanitaria Parc Tauli, Inst Invest Innovacio Sanitaria Parc Tauli 1, Infect Dis Dept, Parc Taruli 1, Barcelona 08208, Spain
来源：
MOLECULAR AND CELLULAR PROBES | 2017年 / 32卷
关键词：
Bartonella; Real-time PCR; Optimization; Zoonoses; CAT-SCRATCH DISEASE; INFECTIOUS-DISEASE; DIFFERENTIATION; ASSAY; HENSELAE; IDENTIFICATION; GRAHAMII; GENE; DNA;
D O I：
10.1016/j.mcp.2016.10.006
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A real-time PCR assay using SYBR Green was optimized to detect those Bartonella that are most frequently described as pathogens. The assay was genus-specific. Sequencing allowed to distinguish species. Assay sensitivity was determined using 10-fold serial dilutions of genomic DNA. Dynamic range was 100 ng-100 fg and sensitivity was 50 copies/reaction. (C) 2016 Published by Elsevier Ltd.
引用
收藏
页码：55 / 59
页数：5
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