Hydroalcoholic extract of Rhodiola rosea L. (Crassulaceae) and its hydrolysate inhibit melanogenesis in B16F0 cells by regulating the CREB/MITF/tyrosinase pathway

被引：72
作者：
Chiang, Hsiu-Mei [1 ]
Chien, Yin-Chih [1 ,2 ]
Wu, Chieh-Hsi [2 ]
Kuo, Yueh-Hsiung [3 ,4 ]
Wu, Wan-Chen [1 ]
Pan, Yu-Yun [1 ]
Su, Yu-Han [1 ]
Wen, Kuo-Ching [1 ]
机构：
[1] China Med Univ, Dept Cosmeceut, Taichung 404, Taiwan
[2] China Med Univ, Grad Inst Pharm, Taichung, Taiwan
[3] China Med Univ, Dept Chinese Pharmaceut Sci & Chinese Med Resourc, Taichung 404, Taiwan
[4] Asia Univ, Dept Biotechnol, Taichung 413, Taiwan
来源：
FOOD AND CHEMICAL TOXICOLOGY | 2014年 / 65卷
关键词：
Rhodiola rosea; Melanin; Tyrosinase; MC1R; CREB; GSK3; beta; MELANIN SYNTHESIS; HUMAN MELANOCYTES; TYROSINASE; KINASE; MICROPHTHALMIA; PIGMENTATION; PERFORMANCE; MECHANISM; SYNTHASE; OXIDASE;
D O I：
10.1016/j.fct.2013.12.032
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
We investigated the effects of an aqueous alcohol extract of Rhodiola rosea (R. rosea) and its hydrolysate on melanin synthesis and the mechanisms mediating the activity. The ratio of tyrosol to salidroside was 2.3 in hydroalcoholic extract, and 51.0 in hydrolysate. We found that R. rosea extract and its hydrolysate inhibited melanin synthesis and tyrosinase activity in mouse melanoma cells (B16F0 cells). R. rosea extract also inhibited gene and protein expression of melanocortin 1 receptor (MC1R) and inhibited c-AMP response element binding protein (CREB) phosphorylation, suppressed the activation of ART and glycogen synthase kinase-3 beta (GSK3 beta), and inhibited the expression of microphthalmia-associated transcription factor (MITF) and tyrosinase-related protein 1 (TRP-1). R. rosea hydrolysate inhibited the phosphorylation of CREB, the activation of AKT and GSK3 beta, and the expression of MITF and tyrosinase. Our results suggest that R. rosea extract is a novel tyrosinase inhibitor and that it exerts its effects by regulating the CREB/MITF/tyrosinase pathway in B16F0. Further in vivo studies are needed to determine the effectiveness of R. rosea extract as a skin whitening agent. (C) 2014 Elsevier Ltd. All rights reserved.
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页码：129 / 139
页数：11
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