Scanning holographic microscopy of three-dimensional fluorescent specimens

被引:64
|
作者
Indebetouw, Guy [1 ]
Zhong, Wenwei [1 ]
机构
[1] Virginia Tech, Dept Phys, Blacksburg, VA 24061 USA
关键词
D O I
10.1364/JOSAA.23.001699
中图分类号
O43 [光学];
学科分类号
070207 ; 0803 ;
摘要
We demonstrate experimentally the three-dimensional reconstructions of fluorescent biological specimens using scanning holographic microscopy. Three-dimensional reconstructions with transverse resolution below about 1 mu m of transmission and fluorescence emission images are presented and analyzed. The limitations of the method are discussed. (C) 2006 Optical Society of America.
引用
收藏
页码:1699 / 1707
页数:9
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