Scanning holographic microscopy of three-dimensional fluorescent specimens

被引：67

作者：

Indebetouw, Guy ^{[1
]}

Zhong, Wenwei ^{[1
]}

机构：

[1] Virginia Tech, Dept Phys, Blacksburg, VA 24061 USA

来源：

JOURNAL OF THE OPTICAL SOCIETY OF AMERICA A-OPTICS IMAGE SCIENCE AND VISION | 2006年 / 23卷 / 07期

关键词：

D O I：

10.1364/JOSAA.23.001699

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

We demonstrate experimentally the three-dimensional reconstructions of fluorescent biological specimens using scanning holographic microscopy. Three-dimensional reconstructions with transverse resolution below about 1 mu m of transmission and fluorescence emission images are presented and analyzed. The limitations of the method are discussed. (C) 2006 Optical Society of America.

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收藏

页码：1699 / 1707

页数：9

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