Development of target delivery system based on actinomycin class drugs and recombinant alpha-fetoprotein

被引：4
|
作者：
Nikolskaya, E. D. [1 ]
Zhunina, O. A. [1 ]
Yabbarov, N. G. [1 ]
Shvets, V. I. [2 ]
Krugliy, B. I. [1 ]
Severin, E. S. [1 ]
机构：
[1] Russian Res Ctr Mol Diagnost & Therapy, Moscow 117149, Russia
[2] Moscow State Univ Fine Chem Technol MITHT, Moscow 119571, Russia
来源：
DOKLADY BIOCHEMISTRY AND BIOPHYSICS | 2017年 / 473卷 / 01期
关键词：
D O I：
10.1134/S1607672917020156
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A recombinant alpha-fetoprotein (rAFP) was obtained in the yeast P. pastoris system, and its functional activity was confirmed. A method for producing polymer particles loaded with dactinomycin was developed, and a conjugate of these nanoparticles with rAFP was synthesized. The efficiency of the obtained conjugate on the HeLa, SKOV3, and MG-63 tumor cells and the absence of toxicity on the normal cells was shown. Experiments in vivo demonstrated a significant increase in the antitumor efficacy of the conjugate at a lower general toxicity as compared to the commercially available dactinomycin.
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收藏
页码：148 / 150
页数：3
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