Appraisal of the biological aspect of Zinc oxide nanoparticles prepared using extract of Camellia sinensis L.

Green synthesis of nanoparticles by plant extracts is receiving enormous attention due to their abundant availability, providing a broad range of eco-friendly and bioactive metabolites. This study was designed to synthesize biocompatible zinc oxide nanoparticles (ZnO-NPs) using the aqueous leaf extracts of the Camellia sinensis L. at 400 and 550 degrees C calcination temperatures. The physicochemical properties, as well as nanometric characterization of the ZnO-NPs, were performed by Energy Dispersive x-ray Spectroscopy (EDS), Field Emission Scanning Electron Microscopy (FESEM), Fourier Transform Infrared Spectroscopy (FTIR), and UV-visible spectroscopy. To achieve nanoparticles for being applied in bioactive molecules delivery applications, biological assays such as in vitro cytotoxicity; antibacterial, hemolytic, anti-oxidant (FRAP and DPPH) activities were done for the synthesized NPs. The results were promising and indicated that both of the prepared NPs displayed reliable outcomes for antibacterial activity and blood biocompatibility assays. Whereas, ZnO-NPs prepared at 550 degrees C had lower cytotoxicity than those prepared at 400 degrees C calcination in MCF-7 and fibroblast cell lines. Our findings demonstrated that the using aqueous leaf extracts of the Camellia sinensis L. for the preparation of ZnO-NPs is an efficient green synthesis procedure and the obtained NPs have acceptable biocompatibility and bioactivity.