Imaging of poly(N-isopropyl acrylamide-co-acrylic acid) amino acid conjugates with scanning tunnelling microscopy

被引:3
作者
Koçum, C
Bulmus, V
Piskin, E [1 ]
机构
[1] Hacettepe Univ, Dept Chem Engn, TR-06532 Ankara, Turkey
[2] Hacettepe Univ, Bioengn Div, TR-06532 Ankara, Turkey
关键词
D O I
10.1106/088391102027132
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
A copolymer of N-isopropyl acrylamide (NIPAAm) and acrylic acid (AAc), Mw 1,400, AAc content of 3.1 mmol carboxylic acid groups per gram of the copolymer, was synthesised by a free radical polymerisation. Mono-, di-, and tripeptide conjugates of this copolymer were prepared by using the carboxyl-protected alanine, glycine and serine, with a water-soluble carbodiimide. Scanning tunnelling microscope images of the conjugates were taken after each synthesis step. The average length and width of the copolymer with no amino acid were measured as 17 and 2.5 nm, respectively. The lengths of the mono-, di-, and tri-peptide conjugates were similar to the length of the copolymer chain while the widths were in the range of 3.0-3.2, 3.5-4.0 and 4.2-4.4 nm, respectively.
引用
收藏
页码:239 / 250
页数:12
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