TCGA whole-transcriptome sequencing data reveals significantly dysregulated genes and signaling pathways in hepatocellular carcinoma

被引：54

作者：

Ho, Daniel Wai-Hung

Kai, Alan Ka-Lun

Ng, Irene Oi-Lin ^{[1
]}

机构：

[1] Univ Hong Kong, Dept Pathol, Hong Kong, Hong Kong, Peoples R China

来源：

FRONTIERS OF MEDICINE | 2015年 / 9卷 / 03期

关键词：

TCGA; whole-transcriptome sequencing; HCC; liver cancer; CELL-CYCLE CONTROL; TUMOR-GROWTH; CANCER; VASCULARIZATION; SUPPRESSOR; BIOMARKERS; PACKAGE; PROTEIN; HCC;

D O I：

10.1007/s11684-015-0408-9

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

This study systematically evaluates the TCGA whole-transcriptome sequencing data of hepatocellular carcinoma (HCC) by comparing the global gene expression profiles between tumors and their corresponding nontumorous liver tissue. Based on the differential gene expression analysis, we identified a number of novel dysregulated genes, in addition to those previously reported. Top-listing upregulated (CENPF and FOXM1) and downregulated (CLEC4G, CRHBP, and CLEC1B) genes were successfully validated using qPCR on our cohort of 65 pairs of human HCCs. Further examination for the mechanistic overview by subjecting significantly upregulated and downregulated genes to gene set enrichment analysis showed that different cellular pathways were involved. This study provides useful information on the transcriptomic landscape and molecular mechanism of hepatocarcinogenesis for development of new biomarkers and further in-depth characterization.

引用

页码：322 / 330

页数：9

共 29 条

[1] Cross-Species Regulatory Network Analysis Identifies a Synergistic Interaction between FOXM1 and CENPF that Drives Prostate Cancer Malignancy [J].