Multiplex Y chromosome SNP genotyping using MALDI-TOF mass spectrometry

被引：2

作者：

Mengel-Jorgensen, J

Sanchez, JJ

Borsting, C

Kirpekar, F

Morling, N

机构：

[1] Univ Copenhagen, Inst Forens Med, Dept Forens Genet, DK-2100 Copenhagen O, Denmark

[2] Univ So Denmark, Dept Biochem & Mol Biol, Odense, Denmark

来源：

PROGRESS IN FORENSIC GENETICS 10 | 2004年 / 1261卷

关键词：

Y chromosome; SNP; MALDI-TOF;

D O I：

10.1016/s0531-5131(03)01544-9

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Matrix-assisted laser desorption/ionisation time-of-flight (MALDI-TOF) mass spectrometry (MS) offers a reliable, inexpensive and time-saving method for SNP genotyping. The method is based on weight determination of single base extended (SBE) primers. Here, we present a MALDI-TOF protocol for typing 17 Y chromosome SNPs using biotin-labelled ddNTP in the SBE reaction and solid-phase avidin capture purification of extended primers. All 17 fragments were amplified in a multiplex reaction and all 17 primers were extended in a single SBE reaction. (C) 2003 Elsevier B.V. All rights reserved.

引用

页码：15 / 17

页数：3

共 7 条

[1]

Fei ZD, 2000, RAPID COMMUN MASS SP, V14, P950, DOI 10.1002/(SICI)1097-0231(20000615)14:11<950::AID-RCM971>3.0.CO

[2]

2-3

[3] Solid phase capturable dideoxynucleotides for multiplex genotyping using mass spectrometry [J].