Molecular characterization and epidemiology of the highly pathogenic avian influenza H5N1 in Nigeria

被引：21

作者：

Fasina, F. O. ^{[1
,2
]}

Bisschop, S. P. R. ^{[2
]}

Joannis, T. M.

Lombin, L. H.

Abolnik, C. ^{[3
]}

机构：

[1] Natl Inst Vet Res, Viral Res Div, Vom, Nigeria

[2] Univ Pretoria, Fac Vet Sci, Poultry Reference Ctr, ZA-0002 Pretoria, South Africa

[3] ARC Onderstepoort Vet Inst, Pretoria, South Africa

来源：

EPIDEMIOLOGY AND INFECTION | 2009年 / 137卷 / 04期

关键词：

Avian influenza H5N1; SEQUENCE ALIGNMENT; VIRUSES; AFRICA; POULTRY;

D O I：

10.1017/S0950268808000988

中图分类号：

R1 [预防医学、卫生学];

学科分类号：

1004 ; 120402 ;

摘要：

Avian influenza caused infection and spread throughout Nigeria in 2006. Carcass samples (lung, liver, spleen, heart, trachea and intestine) from the different regions of Nigeria were processed for virus isolation. Infective allantoic fluids were tested for avian influenza viruses (AIV) and Newcastle disease virus using monospecific antisera. Thirty-five isolates were generated and characterized molecularly using the haemagglutinin gene. The molecular analysis indicated that different sublineages of the highly pathogenic avian influenza (HPAI) H5N1 viruses spread throughout Nigeria. We compared the Nigerian isolates with others from Africa and results indicated close similarities between isolates from West Africa and Sudan. Some of the analysed viruses showed genetic drift, and the implications of these for future epidemiology and ecology of avian influenza in Africa require further evaluation. The spread of primary outbreaks was strongly linked to trade (legal and illegal), live bird markets, inappropriate disposal, and poorly implemented control measures. No strong correlation existed between wild birds and HPAI H5N1 in Nigeria.

引用

页码：456 / 463

页数：8

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