Transfection of the glial cell line-derived neurotrophic factor gene promotes neuronal differentiation

被引：8

作者：

Du, Jie ^{[1
,2
]}

Gao, Xiaoqing ^{[3
]}

Deng, Li ^{[3
]}

Chang, Nengbin ^{[2
]}

Xiong, Huailin ^{[2
]}

Zheng, Yu ^{[1
]}

机构：

[1] Sichuan Univ, West China Sch Preclin & Forens Med, Dept Physiol, Chengdu 610041, Sichuan Provinc, Peoples R China

[2] Luzhou Med Coll, Dept Anat, Luzhou, Sichuan Provinc, Peoples R China

[3] Luzhou Med Coll, Res Ctr Preclin Med, Luzhou, Sichuan Provinc, Peoples R China

来源：

NEURAL REGENERATION RESEARCH | 2014年 / 9卷 / 01期

关键词：

nerve regeneration; bone marrow mesenchymal stem cells; cell differentiation; neuron-like cells; glial cell line-derived neurotrophic factor; recombinant adenovirus vector; transfection; retinoic acid; epidermal growth factor; nerve growth factor; growth-associated protein-43; neural regeneration; MARROW STROMAL CELLS; MESENCHYMAL STEM-CELLS; NERVE GROWTH-FACTOR; INTRACEREBRAL HEMORRHAGE; CEREBRAL-ISCHEMIA; BRAIN; GDNF; RAT; EXPRESSION; MOTOR;

D O I：

10.4103/1673-5374.125327

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Glial cell line-derived neurotrophic factor recombinant adenovirus vector-transfected bone marrow mesenchymal stem cells were induced to differentiate into neuron-like cells using inductive medium containing retinoic acid and epidermal growth factor. Cell viability, microtubule-associated protein 2-positive cell ratio, and the expression levels of glial cell line-derived neurotrophic factor, nerve growth factor and growth-associated protein-43 protein in the supernatant were significantly higher in glial cell line-derived neurotrophic factor/bone marrow mesenchymal stem cells compared with empty virus plasmid-transfected bone marrow mesenchymal stem cells. Furthermore, microtubule-associated protein 2, glial cell line-derived neurotrophic factor, nerve growth factor and growth-associated protein-43 mRNA levels in cell pellets were statistically higher in glial cell line-derived neurotrophic.factor/bone marrow mesenchymal stem cells compared with empty virus plasmid-transfected bone marrow mesenchymal stem cells. These results suggest that glial cell line-derived neurotrophic factor/bone marrow mesenchymal stem cells have a higher rate of induction into neuron-like cells, and this enhanced differentiation into neuron-like cells may be associated with up-regulated expression of glial cell line-derived neurotrophic factor, nerve growth factor and growth-associated protein-43.

引用

页码：33 / 40

页数：8

共 42 条

[1] GDNF prevents degeneration and promotes the phenotype of brain noradrenergic neurons in vivo [J].

Arenas, E ;

Trupp, M ;

Akerud, P ;

Ibanez, CF .

NEURON, 1995, 15 (06) :1465-1473

[2] Engraftment and migration of human bone marrow stromal cells implanted in the brains of albino rats - similarities to astrocyte grafts [J].

Azizi, SA ;

Stokes, D ;

Augelli, BJ ;

DiGirolamo, C ;

Prockop, DJ .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (07) :3908-3913

[3] Neural progenitor cells engineered to secrete GDNF show enhanced survival, neuronal differentiation and improve cognitive function following traumatic brain injury [J].

Bakshi, A ;

Shimizu, S ;

Keck, CA ;

Cho, S ;

LeBold, DG ;

Morales, D ;

Arenas, E ;

Snyder, EY ;

Watson, DJ ;

McIntosh, TK .

EUROPEAN JOURNAL OF NEUROSCIENCE, 2006, 23 (08) :2119-2134

[4] GAP-43: An intrinsic determinant of neuronal development and plasticity [J].

Benowitz, LI ;

Routtenberg, A .

TRENDS IN NEUROSCIENCES, 1997, 20 (02) :84-91

[5] Reporter gene-expressing bone marrow-derived stromal cells are immune-tolerated following implantation in the central nervous system of syngeneic immunocompetent mice [J].

Bergwerf, Irene ;

De Vocht, Nathalie ;

Tambuyzer, Bart ;

Verschueren, Jacob ;

Reekmans, Kristien ;

Daans, Jasmijn ;

Ibrahimi, Abdelilah ;

Van Tendeloo, Viggo ;

Chatterjee, Shyama ;

Goossens, Herman ;

Jorens, Philippe G. ;

Baekelandt, Veerle ;

Ysebaert, Dirk ;

Van Marck, Eric ;

Berneman, Zwi N. ;

Van Der Linden, Annemie ;

Ponsaerts, Peter .

BMC BIOTECHNOLOGY, 2009, 9

[6] Reparative mechanisms in the cerebellar cortex [J].

Carulli, D ;

Buffo, A ;

Strata, P .

PROGRESS IN NEUROBIOLOGY, 2004, 72 (06) :373-398

[7] Neuroprotective effect of grafting GDNF gene-modified neural stem cells on cerebral ischemia in rats [J].

Chen, B. ;

Gao, X. -Q. ;

Yang, C. -X. ;

Tan, S. -K. ;

Sun, Z-L ;

Yan, N. -H. ;

Pang, Y. -G. ;

Yuan, M. ;

Chen, G. -J. ;

Xu, G. -T. ;

Zhang, K. ;

Yuan, Q. L. .

BRAIN RESEARCH, 2009, 1284 :1-11

[8] Analysis of neuron-like differentiation of human bone marrow mesenchymal stem cells [J].

Choi, Chi Bong ;

Cho, Yun Kyoung ;

Prakash, K. V. Bhanu ;

Jee, Bo Keun ;

Han, Chang Whan ;

Paik, Young-Ki ;

Kim, Hwi-Yool ;

Lee, Kweon-Haeng ;

Chung, Namhyun ;

Rha, Hyoung Kyun .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2006, 350 (01) :138-146

[9] Allogeneic bone marrow-derived flk-1+Sca-1- mesenchymal stem cells leads to stable mixed chimerism and donor-specific tolerance [J].

Deng, WM ;

Han, Q ;

Liao, LM ;

Li, CH ;

Ge, W ;

Zhao, ZG ;

You, SG ;

Deng, HY ;

Zhao, RCH .

EXPERIMENTAL HEMATOLOGY, 2004, 32 (09) :861-867

[10] Gene Therapy for Parkinson's Disease [J].

Denyer, Rachel ;

Douglas, Michael R. .

PARKINSONS DISEASE, 2012, 2012

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