Relative biological effectiveness of 25 and 10 kV X-rays for the induction of chromosomal aberrations in two human mammary epithelial cell lines

被引：13

作者：

Beyreuther, Elke ^{[1
]}

Doerr, Wolfgang ^{[2
]}

Lehnert, Anna ^{[1
]}

Lessmann, Elisabeth ^{[1
]}

Pawelke, Joerg ^{[1
,3
]}

机构：

[1] Forschungszentrum Dresden Rossendorf, Inst Radiat Phys, D-01314 Dresden, Germany

[2] Tech Univ Dresden, Dept Radiotherapy & Radiat Oncol, Med Fac Carl Gustav Carus, Dresden, Germany

[3] Tech Univ Dresden, Ctr Radiat Res Oncol, Med Fac Carl Gustav Carus, Dresden, Germany

来源：

RADIATION AND ENVIRONMENTAL BIOPHYSICS | 2009年 / 48卷 / 03期

关键词：

HUMAN PERIPHERAL LYMPHOCYTES; NEOPLASTIC TRANSFORMATION; DICENTRIC CHROMOSOMES; RBE; RADIATION; ENERGY; MICRONUCLEI; MCF-12A;

D O I：

10.1007/s00411-009-0221-4

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Administration of ionizing radiation for diagnostic purposes can be associated with a risk for the induction of tumors. Therefore, particularly with regard to general screening programs, e.g. with mammography, cost-benefit considerations must be discussed including risk estimation depending upon the radiation quality administered. The present study was initiated to investigate the in vitro X-ray energy dependence for the induction of chromosomal aberrations in the two mammary epithelial cell lines, 184A1 and MCF-12A. The induced excess fragments, dicentric chromosomes and centric rings were analyzed and the relative biological effectiveness (RBE) was determined for 10 and 25 kV X-rays relative to 200 kV X-rays. The assumed energy dependence with higher values for 10 kV X-rays was confirmed for the excess fragments, with RBE(M) values of 1.92 +/- A 0.26 and 1.40 +/- A 0.12 for 10 kV X-rays and 1.17 +/- A 0.12 and 0.97 +/- A 0.10 for 25 kV photons determined for cell lines 184A1 and MCF-12A, respectively. Meaningful results for the induction of dicentric chromosomes and centric rings were obtained only for higher doses with RBE values of 1.31 +/- A 0.21 and 1.70 +/- A 0.29 for 184A1 and 1.08 +/- A 0.08 and 1.43 +/- A 0.12 for MCF-12A irradiated with 25 and 10 kV X-rays, respectively.

引用

页码：333 / 340

页数：8

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