miR-215 suppresses proliferation and migration of non-small cell lung cancer cells

被引：28

作者：

Cai, Xiaopan ^{[1
]}

Peng, Dongyu ^{[1
]}

Wei, Haifeng ^{[1
]}

Yang, Xinghai ^{[1
]}

Huang, Quan ^{[1
]}

Lin, Zaijun ^{[1
]}

Xu, Wei ^{[1
]}

Qian, Ming ^{[1
]}

Yang, Cheng ^{[1
]}

Liu, Tielong ^{[1
]}

Yan, Wangjun ^{[1
]}

Zhao, Jian ^{[1
]}

机构：

[1] Second Mil Med Univ, Changzheng Hosp, Dept Bone Tumor Surg, 415 Fengyang Rd, Shanghai 200003, Peoples R China

来源：

ONCOLOGY LETTERS | 2017年 / 13卷 / 04期

关键词：

non-small cell lung cancer; migration; microRNA; lung cancer; miR-215; TUMOR-SUPPRESSOR; POOR-PROGNOSIS; COLON-CANCER; RISK-FACTORS; EPIDEMIOLOGY; DIAGNOSIS; MICRORNA; METASTASIS; PATHWAY; MARKER;

D O I：

10.3892/ol.2017.5692

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The expression of microRNA-215 (miR-215) in non-small cell lung cancer (NSCLC) tissues and the effects of miR-215 on the proliferation and migration of NSCLC cells were investigated. qRT-PCR was used to detect the expression of miR-215 in NSCLC tissues and paired normal tumor-adjacent lung tissues; MTT assay, transwell assay and soft-agar assay were used in vitro to evaluate the role of miR-215 on proliferation, migration and cell clonality on NSCLC cells, after transfecting miR-215 mimics to NSCLC cell line A549 or miR-215 to H1299. miR-215 was significantly decreased in NSCLC tissues compared to the paired normal tissues; Overexpression of miR-215 in A549 cells resulted in reduction of the cell proliferation, migration and cell clonality, while downregulation of miR-215 in H1299 cells could promote cell proliferation, migration and clonality. In conclusion, miR-215 was downregulated in NSCLC tissues and may play a key role in the development of NSCLC.

引用

页码：2349 / 2353

页数：5

共 22 条

[1] RADIOSENSITIZING EFFECTS OF ECTOPIC MIR-101 ON NON-SMALL-CELL LUNG CANCER CELLS DEPEND ON THE ENDOGENOUS MIR-101 LEVEL [J].